Mapping local electric fields in proteins at biomimetic interfaces

Gal Schkolnik; Tillmann Utesch; Johannes Salewski; Katalin Tenger; Diego Millo; Anja Kranich; Ingo Zebger; Claudia Schulz; László Zimányi; Gábor Rákhely; Maria Andrea Mroginski; Peter Hildebrandt

doi:10.1039/C1CC13186A

Mapping local electric fields in proteins at biomimetic interfaces†

Gal Schkolnik,^a Tillmann Utesch,^a Johannes Salewski,^a Katalin Tenger,^b Diego Millo,^a Anja Kranich,^a Ingo Zebger,^a Claudia Schulz,^a László Zimányi,^b Gábor Rákhely,^c Maria Andrea Mroginski^a and Peter Hildebrandt*^a

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* Corresponding authors

^a Technische Universität Berlin, Institut für Chemie, Sekr. PC14, Straße des 17. Juni 135, D-10623 Berlin, Germany
E-mail: hildebrandt@chem.tu-berlin.de
Fax: +49 30 31421122
Tel: +49 30 31421419

^b Institute of Biophysics, Biological Research Centre, H-6726 Szeged Temesv, Hungary

^c Department of Biotechnology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

Abstract

We present a novel approach for determining the strength of the electric field experienced by proteins immobilised on membrane models. It is based on the vibrational Stark effect of a nitrile label introduced at different positions on engineered proteins and monitored by surface enhanced infrared absorption spectroscopy.

Chemical Communications

Mapping local electric fields in proteins at biomimetic interfaces†

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Mapping local electric fields in proteins at biomimetic interfaces

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