Ultrasensitive and selective detection of nicotinamide adenine dinucleotide by target-triggered ligation–rolling circle amplification

Abstract

An ultrasensitive fluorescence assay for nicotinamide adenine dinucleotide (NAD⁺) was developed by target-triggered ligation–rolling circle amplification (L-RCA). This novel approach can detect as low as 1 pM NAD⁺, much lower than those of previously reported biosensors, and exhibits high discrimination ability even against 200 times excess of NAD⁺ analogs.