Issue 53, 2012
From the journal:

Chemical Communications

Preparation and characterization of DNA aptamer based spin column for enrichment and separation of histones†

Nan Shao,ab   Kai Zhang,*ab   Ying Chen,b   Xiwen Heb  and  Yukui Zhangabc  

* Corresponding authors

a State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, P. R. China
E-mail: zhangkai730@yahoo.com.cn
Tel: 86-22-23494962

b Department of Chemistry, Nankai University, Tianjin 300071, P. R. China

c National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China

Abstract

Core histones play a critical role in the regulation of chromatin-templated biological process. Here we have developed a novel approach to enrich histone proteins based on the recognition of a specific aptamer. The method shows good selectivity toward histone proteins and is able to extract histones directly from complex whole cell lysates.

Graphical abstract: Preparation and characterization of DNA aptamer based spin column for enrichment and separation of histones

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Article information

DOI
https://doi.org/10.1039/C2CC32376A
Article type
Communication
Submitted
02 Apr 2012
Accepted
11 May 2012
First published
11 May 2012

Chem. Commun., 2012,48, 6684-6686

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Preparation and characterization of DNA aptamer based spin column for enrichment and separation of histones

N. Shao, K. Zhang, Y. Chen, X. He and Y. Zhang, Chem. Commun., 2012, 48, 6684 DOI: 10.1039/C2CC32376A

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