A solution 17O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase

Shinya Hanashima; Noriko Fujiwara; Kana Matsumoto; Noriyuki Iwasaki; Guo-qing Zheng; Hidetaka Torigoe; Keiichiro Suzuki; Naoyuki Taniguchi; Yoshiki Yamaguchi

doi:10.1039/C2CC36918D

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A solution ¹⁷O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase†

Shinya Hanashima,^ab Noriko Fujiwara,*^c Kana Matsumoto,^a Noriyuki Iwasaki,^d Guo-qing Zheng,^e Hidetaka Torigoe,^f Keiichiro Suzuki,^c Naoyuki Taniguchi^bgh and Yoshiki Yamaguchi*^ab

Author affiliations

* Corresponding authors

^a Structural Glycobiology Team, RIKEN ASI, 2-1 Hirosawa, Wako-shi, 351-0198 Saitama, Japan
E-mail: yyoshiki@riken.jp
Fax: +81 48-467-9620
Tel: +81 48-467-9619

^b Disease Glycoprobe Team, RIKEN-Max Planck Joint Research Center, RIKEN ASI, Saitama 351-0198, Japan

^c Department of Biochemistry, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan
E-mail: noriko-f@hyo-med.ac.jp
Fax: +81 798-46-3164
Tel: +81 798-45-6357

^d Bruker Daltonics K.K., Kanagawa 221-0022, Japan

^e Department of Physics, Okayama University, Okayama 700-8530, Japan

^f Department of Applied Chemistry, Faculty of Science, Tokyo University of Science, Tokyo 162-8601, Japan

^g Disease Glycomics Team, RIKEN ASI, Saitama 351-0198, Japan

^h The RIKEN-Osaka University Alliance Laboratory, The Institute of Scientific and Industrial Research, Osaka University, Osaka 565-0871, Japan

Abstract

Solution ¹⁷O-NMR application to biological macromolecules is extremely limited. We describe here ¹⁷O-NMR observation of the ¹⁷O₂-oxidized cysteine side chain of human Cu,Zn-superoxide dismutase in solution using selective ¹⁷O₂ oxidation. ¹⁷O-NMR with the aid of ¹⁷O-labeling has wide potential to probe the environment and dynamics of oxidizable functionalities in proteins.

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Article information

DOI: https://doi.org/10.1039/C2CC36918D
Article type: Communication
Submitted: 24 Sep 2012
Accepted: 02 Jan 2013
First published: 03 Jan 2013

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Chem. Commun., 2013,49, 1449-1451

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A solution ¹⁷O-NMR approach for observing an oxidized cysteine residue in Cu,Zn-superoxide dismutase

S. Hanashima, N. Fujiwara, K. Matsumoto, N. Iwasaki, G. Zheng, H. Torigoe, K. Suzuki, N. Taniguchi and Y. Yamaguchi, Chem. Commun., 2013, 49, 1449 DOI: 10.1039/C2CC36918D

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