Issue 28, 2013
From the journal:

Chemical Communications

Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection†

Hirokazu Komatsu,ab   Yutaka Shindo,c   Shigehiro A. Kawashima,ab   Kenzo Yamatsugu,ab   Kotaro Okac  and  Motomu Kanai*ab  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: kanai@mol.f.u-tokyo.a.c. jp
Fax: +81-3-5684-5206
Tel: +81-3-5841-4830

b Japan Science and Technology Agency, ERATO, Kanai Life Science Catalysis Project, Japan

c Graduate School of Science and Technology, Keio University, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan

Abstract

The application of a new rhodamine-based fluorescent probe, RH–NH23 and an acyl transfer promoter, PBu3, to Hela cells induced a time-dependent increase in fluorescence in the mitochondria, which was most likely due to acetylation of RH–NH23 with activated acetyl-CoA by the artificial reaction promoter in living cells.

Graphical abstract: Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection

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Article information

DOI
https://doi.org/10.1039/C3CC40616D
Article type
Communication
Submitted
24 Jan 2013
Accepted
19 Feb 2013
First published
19 Feb 2013

Chem. Commun., 2013,49, 2876-2878

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Intracellular activation of acetyl-CoA by an artificial reaction promoter and its fluorescent detection

H. Komatsu, Y. Shindo, S. A. Kawashima, K. Yamatsugu, K. Oka and M. Kanai, Chem. Commun., 2013, 49, 2876 DOI: 10.1039/C3CC40616D

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