Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing

Abstract

A triazole linkage is formed in RNA by untemplated strain-promoted or CuAAC chemical ligation of 3′-azide and 5′-cyclooctyne oligonucleotides under denaturing conditions. Reverse transcriptase reads through these artificial linkages with omission of one nucleotide. These surprising results have implications for RNA isolation, amplification, sequencing and a variety of biological applications.