Process optimization of cellulase production from alkali-treated coffee pulp and pineapple waste using Acinetobacter sp. TSK-MASC

Abstract

The aim of this study was to assess the mixed combination of coffee pulp waste (CPW) and pineapple waste (PW) residues for cellulase production using newly isolated Acinetobacter sp. TSK-MASC in solid state fermentation. Response surface methodology based Box–Behnken design (BBD) was employed to optimize variables such as pH, incubation time, concentrations of CPW and PW. The BBD design investigation showed a sound adjustment of the quadratic model with the experimental statistics. Statistics based 3-D plots were generated to evaluate the changes in the response surface and to understand the relationship between the enzyme yield and culture parameters. The higher production (888 U mL⁻¹) was achieved after 60 h of incubation with 3.0 g L⁻¹ of CPW and PW at pH 7.0.