Issue 22, 2015
From the journal:

Chemical Communications

APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria†

Roderick M. Dirkzwager,a   Andrew B. Kinghorn,a   Jack S. Richardsb  and  Julian A. Tanner*a  

* Corresponding authors

a Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong S.A.R.
E-mail: jatanner@hku.hk

b The Burnet Institute of Medical Research and Public Health, 85 Commercial Road, Melbourne, Victoria 3004, Australia

Abstract

We report the rapid diagnosis of malaria by aptamer-tethered enzyme capture (APTEC) whereby an aptamer captures biomarker Plasmodium falciparum lactate dehydrogenase (PfLDH) then activity is measured colorimetrically. The robust test was sensitive (limit of detection = 4.9 ng mL−1) and could reliably diagnose malaria in clinical blood samples.

Graphical abstract: APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria

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Article information

DOI
https://doi.org/10.1039/C5CC00438A
Article type
Communication
Submitted
16 Jan 2015
Accepted
10 Feb 2015
First published
11 Feb 2015

Chem. Commun., 2015,51, 4697-4700

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APTEC: aptamer-tethered enzyme capture as a novel rapid diagnostic test for malaria

R. M. Dirkzwager, A. B. Kinghorn, J. S. Richards and J. A. Tanner, Chem. Commun., 2015, 51, 4697 DOI: 10.1039/C5CC00438A

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