Solubilisation of a 2,2-diphenyl-1-picrylhydrazyl radical in water by β-cyclodextrin to evaluate the radical-scavenging activity of antioxidants in aqueous media

Ikuo Nakanishi; Kei Ohkubo; Kohei Imai; Masato Kamibayashi; Yasuo Yoshihashi; Ken-ichiro Matsumoto; Kiyoshi Fukuhara; Katsuhide Terada; Shinobu Itoh; Toshihiko Ozawa; Shunichi Fukuzumi

doi:10.1039/C5CC02236C

Solubilisation of a 2,2-diphenyl-1-picrylhydrazyl radical in water by β-cyclodextrin to evaluate the radical-scavenging activity of antioxidants in aqueous media†

Ikuo Nakanishi,*^a Kei Ohkubo,^bc Kohei Imai,^d Masato Kamibayashi,^e Yasuo Yoshihashi,^f Ken-ichiro Matsumoto,^a Kiyoshi Fukuhara,^d Katsuhide Terada,^f Shinobu Itoh,^b Toshihiko Ozawa^g and Shunichi Fukuzumi*^bchi

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* Corresponding authors

^a Radio-Redox-Response Research Team, Advanced Particle Radiation Biology Research Program, Research Center for Charged Particle Therapy, National Institute of Radiological Sciences (NIRS), Inage-ku, Chiba 263-8555, Japan
E-mail: nakanis@nirs.go.jp
Fax: +81-43-255-6819
Tel: +81-43-206-3131

^b Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan

^c ALCA and SENTAN, Japan Science and Technology Agency (JST), Suita, Osaka 565-0871, Japan

^d School of Pharmacy, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan

^e Pharmaceutical Manufacturing Chemistry, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan

^f Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, Japan

^g Division of Oxidative Stress Research, Showa Pharmaceutical University, Machida, Tokyo 194-8543, Japan

^h Department of Bioinspired Science, Ewha Womans University, Seoul 120-750, Korea

ⁱ Faculty of Science and Technology, Meijo University, Shiogamaguchi, Tempaku, Nagoya, Aichi 468-8502, Japan

Abstract

A 2,2-diphenyl-1-picrylhydrazyl radical (DPPH˙) was successfully solubilised in water by β-cyclodextrin (β-CD). DPPH˙/β-CD thus obtained was demonstrated to be a powerful tool to evaluate the antioxidative activity of water-soluble antioxidants, such as ascorbate and Trolox, in aqueous buffer solutions.

Chemical Communications

Solubilisation of a 2,2-diphenyl-1-picrylhydrazyl radical in water by β-cyclodextrin to evaluate the radical-scavenging activity of antioxidants in aqueous media†

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Solubilisation of a 2,2-diphenyl-1-picrylhydrazyl radical in water by β-cyclodextrin to evaluate the radical-scavenging activity of antioxidants in aqueous media

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