Issue 41, 2015
From the journal:

Chemical Communications

A label-free fluorescent molecular switch for a DNA hybridization assay utilizing a G-quadruplex-selective auramine O†

Huiying Xu,a   Fenghua Geng,b   Yongxiang Wang,*b   Maotian Xu,b   Xinhe Lai,b   Peng Qu,b   Yintang Zhangb  and  Baohong Liu*a  

* Corresponding authors

a Department of Chemistry, State Key Lab of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China
E-mail: bhliu@fudan.edu.cn
Fax: +86-21-65641740
Tel: +86-21-65642009

b Key Laboratory of Biomolecule Recognition and Sensing, College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu, China
E-mail: wangyx2006@pku.edu.cn

Abstract

A sensitive and selective assay of DNA is developed by utilizing a signal transduction strategy with the rational redesign of the hairpin structured G-quadruplex molecular switch (G4-MS) assembled using auramine O (AO). By monitoring the changes of the fluorescent signal, we could identify and further quantitatively determine the target DNA in the samples.

Graphical abstract: A label-free fluorescent molecular switch for a DNA hybridization assay utilizing a G-quadruplex-selective auramine O

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Article information

DOI
https://doi.org/10.1039/C5CC02624E
Article type
Communication
Submitted
30 Mar 2015
Accepted
15 Apr 2015
First published
15 Apr 2015

Chem. Commun., 2015,51, 8622-8625

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A label-free fluorescent molecular switch for a DNA hybridization assay utilizing a G-quadruplex-selective auramine O

H. Xu, F. Geng, Y. Wang, M. Xu, X. Lai, P. Qu, Y. Zhang and B. Liu, Chem. Commun., 2015, 51, 8622 DOI: 10.1039/C5CC02624E

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