Hydrazide functionalized monodispersed silica microspheres: a novel probe with tunable selectivity for a versatile enrichment of phosphopeptides with different numbers of phosphorylation sites in MS analysis

Abstract

Hydrazide functionalized monodispersed silica microspheres (HFMSM) were developed for the enrichment of phosphopeptides for the first time. With the aid of the tunable selectivity of HFMSM, global enrichment or fractionation of phosphopeptides with different numbers of phosphorylation sites could be realized by a simple modulation of the concentrations of formic acid in buffers.