SalL, an enzyme that catalyzes the synthesis of SAM from L-methionine and 5′-chloro-5′-deoxyoadenosine, is shown to accept 5′-chloro-5′-deoxythienoadenosine as a substrate and facilitate the synthesis of a synthetic SAM analog with an unnatural nucleobase. This synthetic cofactor is demonstrated to replace SAM in the DNA methylation reaction with M.TaqI.
C. Vranken, A. Fin, P. Tufar, J. Hofkens, M. D. Burkart and Y. Tor, Org. Biomol. Chem., 2016, 14, 6189 DOI: 10.1039/C6OB00844E
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