Issue 23, 2017

Real-time monitoring of conformational transitions of single-molecule histone deacetylase 8 with nanocircuits

Abstract

Using single-molecule approaches, we directly observed the dynamic interaction between HDAC8 and various ligands as well as conformational interconversions during the catalytic reaction. Statistical analysis identified key kinetic parameters, demonstrating that the enzymatic activity is highly sensitive to both minor variations in the ligand structures and small synthetic molecules.

Graphical abstract: Real-time monitoring of conformational transitions of single-molecule histone deacetylase 8 with nanocircuits

Supplementary files

Article information

Article type
Communication
Submitted
14 Dec 2016
Accepted
27 Feb 2017
First published
27 Feb 2017

Chem. Commun., 2017,53, 3307-3310

Real-time monitoring of conformational transitions of single-molecule histone deacetylase 8 with nanocircuits

S. You, J. Froberg, J. Yu, M. Haldar, A. Sedigh, S. Mallik, D. K. Srivastava and Y. Choi, Chem. Commun., 2017, 53, 3307 DOI: 10.1039/C6CC09949A

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