Issue 11, 2018

A fast responsive, highly selective and light-up fluorescent probe for the two-photon imaging of carboxylesterase in living cells

Abstract

A fast responsive and two photon fluorescent probe (HCyNAc) for carboxylesterase (CaE) has been designed based on the D–π–A structure of hemicyanine and naphthalene derivatives. After enzymatic reaction, HCyNAc enabled light-up fluorescence assay of CaE over other biologically-relevant species and enzymes, including ROS. Two-photon imaging of endogenous CaE was confirmed in HeLa cells using HCyNAc under 800 nm NIR excitation.

Graphical abstract: A fast responsive, highly selective and light-up fluorescent probe for the two-photon imaging of carboxylesterase in living cells

Supplementary files

Article information

Article type
Communication
Submitted
18 Jan 2018
Accepted
20 Feb 2018
First published
21 Feb 2018

J. Mater. Chem. B, 2018,6, 1595-1599

A fast responsive, highly selective and light-up fluorescent probe for the two-photon imaging of carboxylesterase in living cells

J. Wang, Q. Chen, N. Tian, W. Zhu, H. Zou, X. Wang, X. Li, X. Fan, G. Jiang and B. Z. Tang, J. Mater. Chem. B, 2018, 6, 1595 DOI: 10.1039/C8TB00147B

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