A naphthalimide-aminal-based pH-sensitive fluorescent donor for lysosome-targeted formaldehyde release and fluorescence turn-on readout

Hang Xu; Shengnan Ma; Qianqian Liu; Lixian Huang; Peimin Wu; Xiaolin Liu; Yali Huang; Xiaolei Wang; Huan Xu; Kaiyan Lou; Wei Wang

doi:10.1039/C9CC02481F

A naphthalimide-aminal-based pH-sensitive fluorescent donor for lysosome-targeted formaldehyde release and fluorescence turn-on readout†

Hang Xu,

^a Shengnan Ma,^a Qianqian Liu,^a Lixian Huang,^a Peimin Wu,^a Xiaolin Liu,^a Yali Huang,^a Xiaolei Wang,^a Huan Xu,*^a Kaiyan Lou

*^a and Wei Wang

*^ab

Author affiliations

* Corresponding authors

^a State Key Laboratory of Bioengineering Reactor, Shanghai Key Laboratory of New Drug Design, and Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science & Technology, 130 Meilong Road, Shanghai 200237, China
E-mail: xuhuan@ecust.edu.cn, kylou@ecust.edu.cn

^b Department of Pharmacology and Toxicology and BIO5 Institute, University of Arizona, Tucson, AZ 85721-0207, USA
E-mail: wwang@pharmacy.arizona.edu

Abstract

A naphthalimide-aminal-based fluorescent donor (NAP-FAD-1) is a pH-sensitive smart donor, which features accelerated FA release at pH 5.0 compared to that at pH 7.4 and fluorescence turn-on readout for facile tracking and quantification of FA release. Confocal imaging studies of HeLa cells co-incubated with Lyso-Tracker Red suggested that its FA release is lysosome-targeted.

Chemical Communications

A naphthalimide-aminal-based pH-sensitive fluorescent donor for lysosome-targeted formaldehyde release and fluorescence turn-on readout†

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A naphthalimide-aminal-based pH-sensitive fluorescent donor for lysosome-targeted formaldehyde release and fluorescence turn-on readout

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