Enhancing the peroxidase-like activity and stability of gold nanoparticles by coating a partial iron phosphate shell

Abstract

Using citrate-capped gold nanoparticles (AuNPs) as peroxidase-mimicking enzymes to design biosensors is hindered by their low catalytic activity and poor colloidal stability, resulting in limited sensitivity and large variations. Herein, the growth of a partial iron phosphate (FeP) shell with Fe²⁺ ions on citrate-capped AuNPs boosted the activity of the AuNPs by up to 20-fold. The FeP-enhanced activity was demonstrated on AuNPs of different sizes, and gold nanostars. When the FeP layer is thick enough to block the access to the Au/FeP interface, the activity was inhibited. Capping the remaining Au surface by thiol also inhibited the activity, suggesting that faster reactions occurred at the interfaces of Au/FeP. Moreover, a FeP shell can stabilize AuNPs against freezing and a high NaCl concentration of 1 M. Sensitive detection of Fe²⁺ was achieved with a detection limit of 0.41 μM, while no other tested transition metal phosphates enhanced the peroxidase-like activity of AuNPs.