Detection of organophosphorus pesticides: exploring oxime as a probe with improved sensitivity by CeO2-modified electrode

Abstract

In this paper, the catalytic activity of CeO₂ NPs toward oxime oxidation was adopted for the first time to develop an electrochemical sensor with improved sensitivity toward the direct detection of organophosphorus pesticides (OPs) without electrochemical redox activity. To enhance the conductivity of the sensor, CeO₂ NPs together with multi-walled carbon nanotubes (MWCNTs) were deposited onto a bare glassy carbon electrode (GCE) by the simple method of drop-casting. The electrochemical properties of the as-prepared sensor were evaluated in K₃[Fe(CN)₆] solution and the oxidation behavior of pralidoxime (PAM) chloride on the electrodes was characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The results show that the modification of CeO₂ onto the electrode not only increases the electroactive area of the electrode but also significantly increases the peak current of PAM chloride oxidation, which confirms that CeO₂ has an electrocatalytic effect toward oxime oxidation. To evaluate the sensitivity of the as-fabricated sensor, the inhibition rate of PAM chloride peak current was tested in PAM chloride solution containing different concentrations of chlorpyrifos, which shows a very small detection limit of 2.5 × 10⁻⁹ M. In addition, the sensor successfully achieved a convenient and sensitive determination of OPs in vegetable extracts.