Issue 42, 2023
From the journal:

Organic & Biomolecular Chemistry

A macrocyclic fluorescent probe for the detection of citrate†

Stephen M. Butler, ORCID logo ab   Maria Hountondji,ab   Stuart N. Berry, ORCID logo a   Jian Tan,cd   Laurence Maciacde  and  Katrina A. Jolliffe ORCID logo *abf  

* Corresponding authors

a School of Chemistry, The University of Sydney, NSW, Australia
E-mail: kate.jolliffe@sydney.edu.au

b Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Sydney, NSW, Australia

c The Charles Perkins Centre, The University of Sydney, NSW 2006, Australia

d School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, NSW 2006, Australia

e Sydney Cytometry, The University of Sydney, NSW 2006, Australia

f The University of Sydney Nano Institute (Sydney Nano), The University of Sydney, NSW 2006, Australia

Abstract

We present a macrocyclic fluorescent probe for the detection of citrate. This receptor binds citrate through hydrogen-bonding interactions in aqueous solutions, and exhibits a turn-on in fluorescence in response to binding. The presence of common biologically relevant dicarboxylate species does not significantly impact the fluorescence response. We have demonstrated the utility of this probe with the staining of murine splenocytes, and identified different basal levels of citrate present in immune cell subsets via flow cytometry analysis.

Graphical abstract: A macrocyclic fluorescent probe for the detection of citrate

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Article information

DOI
https://doi.org/10.1039/D3OB01442H
Article type
Paper
Submitted
08 Sep 2023
Accepted
06 Oct 2023
First published
10 Oct 2023

Org. Biomol. Chem., 2023,21, 8548-8553

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A macrocyclic fluorescent probe for the detection of citrate

S. M. Butler, M. Hountondji, S. N. Berry, J. Tan, L. Macia and K. A. Jolliffe, Org. Biomol. Chem., 2023, 21, 8548 DOI: 10.1039/D3OB01442H

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