FTIR spectrum analysis to predict the crystalline and amorphous phases of hydroxyapatite: a comparison of vibrational motion to reflection

Abstract

Hydroxyapatites were synthesized from calcium carbonate and ortho-phosphoric acid in amorphous and crystalline phases by varying sintering temperature from 300 to 1100 °C maintaining an increment of 200 °C. The asymmetric and symmetric stretching, and bending vibrations of phosphate and hydroxyl groups were explored in Fourier transformation infrared (FTIR) spectra. Although the FTIR spectra revealed identical peaks in the full range (400–4000 cm⁻¹ wavenumber), the narrow spectra exerted variations by splitting peaks and intensity. The intensities of peaks at 563, 599, 630, 962, 1026, and 1087 cm⁻¹ wavenumbers were intensified gradually with the augmentation of sintering temperature, and the relation between the relative peak intensity and sintering temperature was correlated with the aid of the good linear regression coefficient. Peak separations were also found in the case of 962 and 1087 cm⁻¹ wavenumbers when the sintering temperature was equal to or exceeded 700 °C. The conventional X-ray diffraction (XRD) technique was also employed to explore the crystalline and amorphous phases of synthesized hydroxyapatites.