Multi-site enzymatic repairing amplification (MSERA) enables ultrasensitive detection of terminal deoxynucleotidyl transferase activity

Abstract

A novel multi-site enzymatic repairing amplification strategy is developed for high-sensitive terminal deoxynucleotidyl transferase quantification through combining enzymatic repairing amplification and lesion base-involved terminal extension. This method may provide a sensitive and flexible tool for molecular diagnosis and drug discovery.