Issue 30, 2010
From the journal:

Chemical Communications

Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution†‡

Weng Lin Tang,ab   Zhi Li*b  and  Huimin Zhao*a  

* Corresponding authors

a Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Avenue, Urbana, USA
E-mail: zhao5@illinois.edu
Fax: +1-217-333-5052
Tel: +1-217-333-2631

b Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117576
E-mail: chelz@nus.edu.sg
Fax: +65-67791936
Tel: +65-65168416

Abstract

We report the first example of directed evolution of a P450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. The biohydroxylation product of the best mutant 1AF4A has an ee of 83% (R) compared to the wild type's ee of 43% (S).

Graphical abstract: Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

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Article information

DOI
https://doi.org/10.1039/C0CC00735H
Article type
Communication
Submitted
02 apr 2010
Accepted
13 mei 2010
First published
03 jun 2010

Chem. Commun., 2010,46, 5461-5463

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Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

W. L. Tang, Z. Li and H. Zhao, Chem. Commun., 2010, 46, 5461 DOI: 10.1039/C0CC00735H

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