Bastian
Bernhardt‡
,
Markus
Schauermann‡
,
Ephrath
Solel
,
André K.
Eckhardt§
and
Peter R.
Schreiner
*
Institute of Organic Chemistry, Justus Liebig University, Heinrich-Buff-Ring 17, 35392 Giessen, Germany. E-mail: prs@uni-giessen.de
First published on 17th November 2022
The search for methods to bind CO2 and use it synthetically as a C1-building block under mild conditions is an ongoing endeavor of great urgency. The formation of heterocyclic carbene–carbon dioxide adducts occurs rapidly when the carbene is generated in solution in the presence of CO2. Here we demonstrate the reversible formation of a complex of the hitherto unreported aminomercaptocarbene (H2N––SH) with CO2 isolated in solid argon by photolysis of 2-amino-2-thioxoacetic acid. Remarkably, the complex disappears in the dark as deduced by time-dependent matrix infrared measurements, and equilibrates back to the covalently bound starting material. This kinetically excluded process below ca. 8 K is made possible through heavy-atom quantum mechanical tunneling, as also evident from density functional theory and ab initio computations at the CCSD(T)/cc-pVTZ level of theory. Our results provide insight into CO2 activation using a carbene and emphasize the role of quantum mechanical tunneling in organic processes, even involving heavy atoms.
Here we present the preparation and reaction of a novel carbene, namely aminomercaptomethylene (1, H2N––SH) in its complex with CO2 (1-CO2) that reacts back to 2-amino-2-thioxoacetic acid (2) under cryogenic conditions (Scheme 1). The concomitant transfer of a proton onto the CO2 moiety leads to a neutral system instead of a zwitterion as in case of the azolium carboxylates, and this process is associated with a very low barrier.
Additionally, we demonstrate that the association reaction is accelerated through heavy-atom quantum mechanical tunneling (QMT) that opens new possibilities for affecting the reactions of CO2 with carbenes or other nucleophiles. In this context we demonstrate the first evidence for 1, the parent structure of nature's thiazol-2-ylidene active site in, e.g., thiamine (vitamin B1) and pyruvate decarboxylase,25 in its complex with CO2. Coincidently, 1-CO2 resembles a rare example of a spectroscopically characterized member of the family of mercaptocarbenes (R––SH) of which hydroxymercaptomethylene was the first spectroscopically identified member.26 Spectrometric evidence has been reported for parent mercaptomethylene.27,28
Under cryogenic conditions the contribution of QMT to the overall reaction rate is larger compared to ambient conditions and sometimes completely determines the qualitative outcome of a reaction.29–31 While proton tunneling is a rather common feature, heavy-atom QMT is encountered less frequently.32–35 However, sometimes even larger groups are transferred as in the case of trifluoroacetyl nitrene, which reacts to trifluoromethyl isocyanate by transferring the CF3-group in a formal [1,2]-shift via QMT.36
High-vacuum flash pyrolysis (HVFP) of α-keto carboxylic acids gives rise to the corresponding hydroxycarbenes that have been investigated via matrix isolation spectroscopy.31,37–43 Besides their intriguing QMT behavior, some hydroxycarbenes add to carbonyls in nearly barrierless carbonyl–ene reactions.44 The reaction 1t-CO2 → 2c (Scheme 1) resembles another example of this reaction type. In analogy to these studies, we used 2 as the precursor for the generation of 1 complexed with CO2.
Fig. 1 Experimental matrix-IR difference spectrum (B) of spectra measured before and after 4 min of irradiation at 254 nm compared with the anharmonic spectrum of 1t-CO2 (A) and 2c (C) computed at the B3LYP/6-311++G(3df,3pd) level of theory (anharmonic). Increasing bands not assigned here are discussed in the ESI.† (Inset) Spectra recorded before (black) and after (red) keeping the matrix in the dark for 70 h. Other time-dependent band profiles are shown in Fig. S14–S23.† |
Upon UV irradiation there are several conceivable reaction paths of 2c. Photoinduced rotamerizations of carboxylic acids46–50 and isomerizations of thioamides to thiolimines51–62 are well known under cryogenic conditions. As 2c contains both of these functionalities, many photoproducts can be envisaged, e.g., the higher lying conformer 2t and 16 conformationally distinct thiolimines, which are 11.4 to 34.4 kcal mol−1 higher in energy than 2c (B3LYP/6-311++G(3df,3pd), see the ESI† for an energetic ranking (Fig. S43†) and experimental data of the rotamerization (Fig. S24†) and tautomerization (Fig. S27†) of 2). Furthermore, we observed the formation of a complex of trans-1 and CO2 (1t-CO2) evidenced by a characteristic matrix infrared (IR) band with maxima at 2336.1, 2333.2, and 2330.4 cm−1 in good agreement with the computed antisymmetric CO2 stretching vibration at 2356.7 cm−1 (B3LYP/6-311++G(3df,3pd), anharmonic) in the complex. Weaker bands at 3444.9 (computed: 3404.6), 1634.8 (1633.0), and 641.8 (625.3) cm−1 can be assigned to 1t-CO2 as well. These bands reach their maximum intensity after 4 min of irradiation at 254 nm (Fig. 1). The assignment is further supported by comparing experimental and computed shifts of perdeuterated 1t-CO2-d3 (ESI, Table S2†).
Much to our surprise, once generated, 1t-CO2 converts back to 2c in the dark. The half-life (t1/2) of this process depends on the matrix site63 and can be derived by monitoring the time-dependent band profile of the antisymmetric CO2 stretching vibration of 1t-CO2. The decay of the maximum at 2336.1 cm−1 yields t1/2 = 26 min (3 K) while the maximum at 2333.2 cm−1 yields t1/2 = 3.8 d (20 K, no reaction at 3 K). The third maximum (2330.4 cm−1) cannot be reliably evaluated due to its small intensity and long half-life. Distinct matrix sites presumably lead to different distances between the two fragments in 1t-CO2, which result in different half-lives. The first value is in excellent agreement with CVT/SCT//B3LYP/6-311+G(d,p) computations yielding t1/2 = 55 min at 3 K for the 1t-CO2 → 2c reaction while the second value agrees well with CVT/SCT//B3LYP/6-311++G(3df,3pd) computations (t1/2 = 7.6 d). The C–C distance in 1t-CO2 is 2.971 Å at the first and 3.005 Å at the latter level of theory; the activation barriers towards 2c are reduced to 1.9 and 2.2 kcal mol−1, respectively. For details on the kinetic analyses see the ESI.†
Even though the computed barrier of 4.2 kcal mol−1 (CCSD(T)/cc-pVTZ) is low, the 1t-CO2 → 2c reaction cannot occur thermally at 3 K, and only QMT explains the experimental observation. To ensure that there is no activation by the spectrometer's light source we repeated the experiment measuring every 5 min while the matrix was not exposed to the spectrometer globar beam between measurements. We also prepared perdeuterated 1t-CO2-d3 whose half-life extends to t1/2 = 36 min (3 K) in the first and t1/2 = 5.1 d (20 K) in the second matrix site (see the ESI† for details). This is in good agreement with CVT/SCT//B3LYP/6-311++G(3df,3pd) computations (t1/2 = 259 min at 3 K, second matrix site: 7.6 d at 20 K). This effect is small (KIE = 1.4 at 3 K, computed: 4.7 at 3 K) owing to the minute movements of the H/D atoms in the QMT process (vide infra). Additionally, we performed kinetic measurements at temperatures between 3 K and 12 K to solidify the QMT mechanism of the reaction 1t-CO2 (2336.1 cm−1) → 2c (Fig. 2). Note that at 20 K we could not detect 1t-CO2 in this matrix site presumably due to its very fast reaction (t1/2 < 1 min).
The logarithmic rate vs. inverse temperature plots (Arrhenius plots) of theory and experiment in Fig. 2 agree well with the regions of Arrhenius and non-Arrhenius behavior, underlining our QMT hypothesis. We conclude that at temperatures below ca. 8 K QMT dominates this reaction entirely. Above ca. 20 K the rate grows exponentially since the barrier of 4.2 kcal mol−1 (CCSD(T)/cc-pVTZ) can be overcome thermally. This system allows for measuring of the kinetics up to temperatures that are in the transition range between the QMT-dominated and the thermally-dominated regions.
Our result can be rationalized comparing the geometry of 1t-CO2 with that of TS_CO2 (point B; Fig. 3). In TS_CO2 the S–H bond does not elongate; the distance the hydrogen atom moves is the result of the H–S–C angle decreasing. Instead, the main movement in TS_CO2 is the two carbon atoms approaching each other by about 0.7 Å. Two hydrogen bonds form between the thiol- and the amino-group facilitating the bonding and activation of CO2. Upon C–C-bond formation the curve flattens and reaches point C (Fig. 3B) corresponding to a zwitterionic structure similar to the carboxylate products in reactions of NHCs with CO2 (cf.Scheme 1). However, point C is not a minimum on the PES (υi = 164.5 cm−1, B3LYP/6-311++G(3df,3pd)) and a hydrogen shift immediately occurs yielding an uncharged species. The potential of this hydrogen transfer is steep and this step does not contribute to the observable kinetics of the reaction. Hence, the measured kinetics (Fig. 2) are due to the two fragments 1 and CO2 approaching each other and not the subsequent hydrogen transfer. Therefore, below ca. 8 K the reaction mechanism can be best described as heavy-atom QMT.
Carbene 1 possesses a singlet ground state and the vertical (adiabatic) singlet/triplet energy separation amounts to 59.4 (38.1) kcal mol−1 at the B3LYP/6-311++G(3df,3pd) level of theory. In 1t-CO2 these values are 61.8 (57.2) kcal mol−1. Complex 1t-CO2 is stabilized by 4.2 kcal mol−1 (CCSD(T)/cc-pVTZ) compared to the free fragments. A bond critical point analysis (Fig. 3, inset) of 1t-CO2 suggests hydrogen bonding interactions (green) between the amino group and CO2 as well as an onset of interactions between the carbon atoms, even at a distance of nearly 3 Å. This leads to a circular arrangement of bonding interactions indicated by a ring critical point (red). The attractive interaction can be interpreted by electron donation from the carbene lone pair to the π*-CO2 orbital (Fig. S38†).
Complexes of carbenes with CO2 might represent transient intermediates in carbene mediated CO2 activation in general. We theoretically found complexes of aminomethylene,64 dihydroxymethylene,38 and aminohydroxymethylene65 with CO2 to be minimum structures on their PES. The carbonyl–ene reactions of aminomethylene and dihydroxymethylene are barrierless while the CO2 addition of aminohydroxymethylene is associated with an activation barrier of 3.9 kcal mol−1 (CCSD(T)/cc-pVTZ). However, these complexes have not been observed experimentally since the mentioned carbenes have been generated under HVFP conditions in the gas phase, when entropy precludes their formation.
As noted above, a CO2 complex of thiazolylidene has been spectroscopically identified earlier, but the back reaction, i.e., CO2 activation has not been reported. We reproduced these results and also found no evidence for the reverse reaction to take place even upon annealing the matrix to 32 K. Note that in thiazolylidene the proton has to be transferred from the NH group (and not from the SH moiety as in 1t-CO2). This possibility is in principle also given in 1t-CO2viaTS15 (Fig. 4).
Fig. 4 IRC curves for the H-transfer in 1t-CO2 from the SH group and the NH2 group (blue) compared to the reaction profile of 13 (red). All IRC curves computed at B3LYP/6-311++G(3df,3pd). |
While the H-transfer from SH is barrierless (Fig. 4, blue), in both cases transfers from NH feature a second barrier after the formation of the zwitterion (2* and 13*). In the case of thiazolylidene the formation of the zwitterion itself is even endothermic. This leads to a large barrier integral and QMT cannot take place. Accordingly, only for the reaction of 1t-CO2 to 2c tunneling was observed.
While NHCs readily react with CO2 in solution to form stable carboxylates, this is not possible in the gas phase or in inert gas matrices due to the charge separation. In 1t-CO2 the thiol group facilitates the formation of a covalent bond by avoiding charge separation through an H shift. This, together with the heavy-atom tunneling uncovered here, opens new avenues for reactions for the activation of small molecules, in particular, CO2.
A related mechanism, albeit thus far not considered may also operate in the initial steps of the conversion of CO2 to formic acid, catalyzed by 1,2,3-triazole.66
Footnotes |
† Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2sc05388h |
‡ These authors contributed equally. |
§ Present address: Lehrstuhl für Organische Chemie II, Ruhr-Universität Bochum, Universitätsstraße 150, 44801 Bochum, Germany. |
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