Shalini
Singh
a,
Neha
Kumari
a,
B. K.
Kanungo
b and
Minati
Baral
*a
aDepartment of Chemistry, National Institute of Technology, Kurukshetra, Haryana 136119, India. E-mail: minatibnitkkr@gmail.com
bDepartment of Chemistry, Sant Longowal Institute of Engineering and Technology, Longowal, Punjab-148106, India
First published on 6th May 2024
Among the currently developed analytical tools, sensors based on fluorescence detection have received immense recognition owing to their high sensitivity, low cost, fast response, and simplicity. The design and synthesis of fluorescence chemosensors to sense metals that are of environmental and biological relevance are of appreciable interest. The efficacy of fluorescent sensors relies on two crucial features: a metal binding unit and a fluorophore that can absorb and emit light. The electronic structure of the sensor is altered upon complexation, leading to a change in light emission or absorption intensity and wavelength. Hydroxypyridinones, a class of N-heterocyclic metal chelators, are appreciated as magnificent chemical tools in metal chelation with a higher affinity towards hard metals, displaying various medical, biological, and industrial applications. However, such compounds are scarcely used as sensors. This article outlines the recent invention of fluorescence chemosensors related to hydroxypyridinone based chelators for the selective sensing of analytes of biological and environmental importance. This discussion involves the structural parameters, coordination mode, and other approaches that helped develop highly selective fluorescence sensors for the ions. In addition, the luminescence properties of the hydroxypyridinones in the energy transfer process of lanthanide chelates as sensitizers are determined.
HOPO-based chelators are recognized for their outstanding chelation properties and their selectivity towards a range of metal ions. These chelators possess a prominent effect on sensitivity and selectivity to the target metal ions by allowing the attachment of a HOPO unit which produces a five-membered stable ring. The selectivity and sensitivity of any chelator towards metal ions can be affected by various parameters including ring size, ionic radii, hardness and softness character, attainment of stability and (or) rigidity, etc. The coordination number, net ionic charge, and stereochemistry of the molecules are among some of the important parameters that are mainly considered while designing a chelator. The selectivity of hydroxypyridinone-based chelators for specific metal ions is justified by introducing the concept of “hard” or “soft” acids and bases. Having two hard oxygen donors for coordination, HOPO-based ligands possess high affinity towards hard metal ions. The design of new multidentate chelators demonstrating high selectivity and sensitivity is based on the factors described above, in which two or more HOPO units are encased to satisfy the desired coordination number of metal ions, forming a number of stable chelating rings (chelate effect) that ultimately provides a topology that minimizes steric requirement. The design of these chelators can be modified by introducing extra functionalization of a chelating moiety with another group to improve their efficacy for sensing purposes and interaction with biological sites.
However, the limitations of HOPO-based compounds towards fluorescence sensing and sensitization arise majorly due to their low solubility in most common organic solvents and water media causing them to lack biocompatibility. Further, low quantum yield and comparatively lower photo stability, together with high detection limits, could be the additional constraints that further limit the compounds for efficient sensing. These shortcomings have challenged researchers for new generations of hydroxypyridinones by refining the molecular design for an enhanced selectivity and biocompatibility that could ensure a higher ligand solubility and provide excellent analytical methods for metal detection and quantification with lower detection limits and higher sensitivity. HOPOs are known for their selectivity for Fe3+ due to their higher pFe3+ values among other dioxo-bidentate ligands.4 Various other HOPO-based ligands have been reported to form stable complexes with a broad range of metal ions including Al3+, Ga3+, Zn2+, and Mg2+ along with lanthanides and actinides. Due to the outstanding iron chelation properties of HOPOs, they have been widely used to treat systematic iron overload diseases, including secondary hemochromatosis and hereditary hemochromatosis.5 Some HOPO-based ligands have been developed for biological applications following the approval of 3-hydroxy-1,2-dimethyl-4-hydroxypyridinone (deferiprone) as an orally active chelating agent to remove excess iron.6 HOPOs and their derivatives are known for displaying various pharmacological properties like antifungal, antibacterial, anticancer, antioxidant, and anticonvulsant activities, which play a great role in drug design and discovery.7,8 They have been immensely used as chelators for diseases like β-thalassemia and other issues linked to the nervous system.9,10 HOPOs possess clinical applications pertaining to their great affinity towards various biologically relevant metal ions.11 They possess good affinity for many transition metals, including Fe(II), Fe(III), Zn(II), Cu(II), etc. HOPO-based chelator antimicrobial agents have shown better activity towards Gram-negative bacteria and Acinetobacter sp.12 Combining HOPOs with antibiotics was also evaluated for potential clinical application.13 Synthesis of mono-HOPO derivatives as potential tyrosinase inhibitors has also been reported.14 HOPO-based metal chelators have also been proven to block virus replication by interacting with the catalytic site of various enzymes.15,16 Cu2+ complexes of HOPO displayed significant activity against M. tuberculosis.17 The antimalarial activity of HOPO derivatives has also been investigated at various times.18 Some work on the coordination properties of hydroxypyridinone and hydroxypyranone-based chelators has been reported from our laboratory.19–21 The majority of the work on HOPOs describes their pharmacological applications. Because of these applications, the importance of working on HOPOs has increased with time. Searching ‘hydroxypyridinone’ as the keyword returned 2358 results in Scopus till 27 November 2023; the inclusion of ‘sensor’ limited the results to 354, and further confining the search to “review” document type gave only 76, whereas no review was available as fluorescence sensor. The latest review on hydroxypyridinone was reported in 2022.22 Most reviews focused on the therapeutical applications of HOPOs and other aspects. A review completely dedicated to the sensing properties has not been compiled despite the availability of several papers on the sensing aspect. A chemosensor is generally described as a “molecule of abiotic origin signaling the presence of matter or energy”.23 A receptor is part of a chemosensor that selectively binds with the analyte which leads to a change in the color, structure, fluorescence or electronic properties of the chemosensor. Therefore, chemosensors can sense the analyte and generate an optical or electrical signal. They can be classified into various types, including electrochemical, colorimetric, and fluorimetric sensors. Nowadays, the area of chemosensors is focused on due to their photophysical properties that are susceptible to the environment. They are extensively used to detect heavy metal ions.24 Although metal ions like Cd2+, Zn2+, Hg2+, Al3+, Cu2+ and Fe3+ are necessary for various essential processes, including oxygen transport, energy production, etc., these ions can be dangerous to human life if appropriate concentrations are not maintained, leading to diseases like Alzheimer's disease, Wilson's disease, and Minamata disease.25–27 Metal ion recognition has captured enough attention in the past decades concerning its applications in biology, the environment, green chemistry, catalysis, etc.28,29 Considerable efforts have been made to synthesize versatile yet easy-to-make sensors with high efficiency and sensitivity for different metal ions. The rising demand for chemosensors is due to their selectivity towards various harmful analytes that can be toxic to human beings; hence, they are useful for environmental and industrial sample analysis for monitoring their concentrations. Among other sensing techniques, fluorescent chemosensors are outstanding due to their versatile properties like high temporal and spatial resolution at a very low concentration, thereby playing an important role in in vivo imaging applications with high sensitivity and selectivity, quick response and good efficiency with a lower maintenance. They generate a fluorescent signal upon altering the photophysical properties upon binding a specific analyte to a receptor;30 there could be an enhancement (turn-on chemosensor) or a decrease (turn-off chemosensor) in fluorescence intensity. Functionalized fluorophore-based analytical assays are important in biotechnology, medicinal chemistry, and environmental sciences.31,32 Fluorescent sensors usually have two major parts: a receptor unit and a fluorophore. The fluorophore transforms information into an optical form by acting as a signaling unit while the receptor recognizes the analyte (Fig. 2). A change in the fluorophore photophysical properties is noticed upon binding of an analyte to the receptor in terms of an optical signal showing enhancement or quenching of fluorescence.33 Recent developments in fluorescent sensor synthesis and application have become a hotspot in opening up new possibilities for sensing and imaging in various industries, including environmental monitoring, biomedical research, and clinical diagnostics.
HOPO-based fluorescent probes have marked their significant use in detecting intracellular metal ions related to various diseases with metal ion burdens. Lanthanides and actinides are universal in contemporary life and play an essential role in various human-oriented activities, like catalysis, organic light-emitting diodes (OLEDs), clean energy technologies, and lasers.34–37 Medical applications including Gd(III)-based contrast agents are being used in MRI and β-emitting 177Lu, used for the treatment of gastroenteropancreatic neuroendocrine tumors as approved by the United States Food and Drug Administration.38,39 Luminescent bio-probes comprising lanthanide complexes impart significant advantages in time-resolved bio-sensing by eliminating background autofluorescence.40 HOPO-based metal binding moieties bear important characteristics of constructive luminescence sensitization of some lanthanide ions and actinides.41 The emission properties of these hydroxypyridinone-based chelators are influenced by various fluorescence mechanisms. The quenching mechanism in most of the HOPO-based fluorescent probes is explained due to the formation of metal chelates; this mechanism is termed chelation-enhanced quenching (CHEQ).42,43,48 Other mechanisms include aggregation-caused quenching (ACQ),84 static quenching mechanism,49 self-quenching mechanism operant in a closely packed solid,81 electron transfer (PET) process inducing intramolecular fluorescence quenching60 and energy transfer mechanism.58 The present review summarizes important chemosensors based on HOPO chelating moieties developed until November 2023 for selective detection of various metal ions. Although much literature is available on hydroxypyridinones, there is less focus on the emission properties of HOPO derivatives and their complexes. The present review concentrates on the sensory aspects of HOPO-based probes and reports various fluorescent and luminescent probes for the selective determination of metal ions, including Fe2+, Cu2+, Mg2+, Zn2+, Fe3+, Gd3+, Tb3+, Eu3+, Cm3+ and Sm3+, as well as anions like phosphate and cyanide. The present review provides insight on the research done in the field of fluorescent chemosensors based on hydroxypyridinone binding moieties. Various types of information regarding their emission properties, quenching mechanisms, detection limits, response time, etc. have been summarized in this paper. Important applications of hydroxypyridinones due to their selective sensing, versatile chelation, and pharmacological properties are detailed with representative examples. This review has been drafted to comprehend the prominence of the design of the molecule leading for various assets including precise sensing, chelation, stability, and biocompatibility. It will pave the way for different approaches for the design of efficient molecular probes by introducing extra functionalities that provide an improved sensing property and thus can be employed for practical applicability such as in cell imaging, real water sample analysis, etc. Taking the above into consideration will result in the development of more significant selective and sensitive chemosensors. The authors believe that the present compilation will help researchers develop new potential compounds incorporating HOPO-based chelating moieties and find them useful for various applications.
Fig. 4 A representation of the profile of fluorescence emission of probe 2 upon titration with an increase in iron concentration.48 |
The loss of fluorescence was observed in the synthesized probes after iron coordination. Further, the pFe3+ value of the fluorescent probes confirms the iron chelation. The quantum mechanical calculation methods indicate the more effective binding ability of the probe based on pyridinone than the pyranone-based one.
Non-transferrin-bound iron (NTBI) is defined as the free iron present in plasma, which is formed due to the increasing saturation of transferrin.50 Two problems are related to its presence, the first being the high affinity of transferrin for iron, which results in low levels of lower molecular weight iron complexes in plasma. Consequently, bacterial infections can be seen more often in iron overload patients as iron is required by the pathogenic bacteria for its growth and replication, and this microbial effect is lost upon saturation of transferrin. Secondly, a distinct uptake system is lacking in NTBI, and transferrin-bound iron is directed to transferrin receptor-expressing cells.51 Ma and the group presented a fast and easy solution for NTBI measurement in serum.52 A fluorescent dye attached to a chelating moiety was designed for iron sensing. A systematic investigation was done for effective chelation using different types of chelators like bidentate hydroxypyridinone (7), bidentate hydroxypyranone (8), hexadentate hydroxypyridinone (9), and hexadentate hydroxypyranone (10), the structures of which are shown in Fig. 5. Competition studies revealed the capability of HOPO-based hexadentate beads to scavenge maximum low molecular mass and albumin-bound iron. The probes were linked to beads to avoid serum samples' autofluorescence, and due to the difference in the size between serum proteins and beads, they could be separated and thus excluded from measurement. The quenching of chelating beads' fluorescence in the presence of iron confirmed the sensitivity towards iron. In short, an assay was developed for the NTBI measurement using an Fe-sensitive probe attached to beads. The reported method can potentially be used to record the progress of treatment of thalassemia, bone marrow transplantation, sickle cell anemia, and hemochromatosis.
Biesuz et al. described the synthesis of a novel chelating solid phase based on silica, which was obtained by the reaction of N-(3′-aminopropyl)-3-hydroxy-2-methyl-4-pyridinone (AHP) with mesoporous silica MCM41 and was modified by reaction with (3-glycidyloxypropyl)trimethoxysilane, for Fe3+ sensing.53 AHP was selected because of the presence of a substituent group of alkyl amine. MCM41, due to its reduced swelling, good mechanical strength, and insolubility in an aqueous medium, was chosen as the solid support. AHP-MCM41@, a new solid-state device, has a total concentration of active sites around 0.4–0.5 mmol g−1 in the final material. The color of the solid phase changes to orange-brown upon complexation with trivalent iron. The equilibrium exists at different M:L stoichiometries between the species and is reached within about two hours, inferring fast sorption kinetics. Mimicking of the behavior of AHP molecules in solution by AHP-MCM41@ was shown upon spectral analysis of the solid phase in equilibrium with solutions of different Fe3+ concentrations. The color change was obtained in the case of AHP-MCM41@ upon coming into contact with Fe3+ in the presence of powerful complexing agents and at neutral pH, making it suitable for Fe3+ sensing (Fig. 6).
Fig. 6 (a) Colors shown for suspended AHP-MCM41@ at different Fe3+/L ratios. (b) Visible spectra of the solid collected in (a).53 |
Ten different Fe-specific fluorescent probes [11(a–j), Fig. 7] were synthesized in which a part of the fluorescent unit was formed by chelating function, making the design of these probes capable of quickly penetrating membranes.54 Quenching of fluorescence occurred upon adding either equimolar Fe3+ or Fe2+, making the probe iron sensitive and potentially measuring the intracellular labile iron pool.
Complete fluorescence quenching was noted in the 3:1 molar ratio [chelator:Fe(III)] for all the chelators while the quenching was off where the molar ratio > 3:1 [chelator:Fe(III)]. At the same proportion, 11(a) showed less sensitive behavior toward other metals like Ni2+, Co2+ and Mn2+. An increase in fluorescence intensity of this probe was observed with metal ions like Zn2+, Ca2+, and Mg2+ [Fig. 8]. In the presence of equimolar Zn2+, the fluorescence of 11(a) was increased with a shift in maximum λem from 464 nm to 430 nm. There was a further rise in fluorescence with increased Zn2+ concentration to 1 mM with the same maximum emission wavelength. The lower ionization constants were indicated by the pFe3+ and pKa values of these ligands, thus showing tight iron binding. Similar pFe3+ values were observed for 11(a) and 11(b), while these values were slightly higher for ligands containing conjugated rings [11(c)–(f)]. The value for 11(g) decreases upon introducing the carboxyl group on the ring, whereas 11(h) possesses the highest pFe3+ values.
Fig. 8 Quenching of 11(a) fluorescence for various metal ions.54 |
A novel fluorescence assay for pFe3+ determination was reported by Ma and co-workers,55 based on the correlations in the presence of iron and the probe between the relative fluorescence and pFe3+. The fluorescent ligand CP645 possesses pFe3+ values within the range of 17–23. The study was expanded, in which the fluorescent probe CP691 (Fig. 9) containing a hexadentate hydroxypyridinone was introduced.56 A maximum emission wavelength at 474 nm was noted for iron-free CP691. The presence of iron quenched the fluorescence intensity of CP691, and an excess addition of potent non-fluorescent ligands could recover this fluorescence change. Twelve non-fluorescent chelators with pFe3+ values within the range ∼20–31 were chosen for the correlation study. As per the thermodynamic considerations, iron could be mobilized from the iron–CP691 complex by the ligands with higher pFe3+ values, leading to a more significant fluorescence recovery. Less than 5% of the fluorescence was recovered using weak ligands like EDTA, rhodotorulic acid, and rhizoferrin as the competing ligands at an equivalent amount to CP691. The pFe3+ values within the range of 24.5–30.5 using the stronger iron affinity probe CP691 achieved a linear fluorescence response.
In further work, selective dual fluorescent iron chelators incorporated in mitochondria-targeted peptides have been designed for the mitochondrial LI pool as biosensors where metal chelating centers and the fluorophore were present on the same structural unit.58 This fluorescent iron chelator possessed an improved molar extinction coefficient and quantum yield as compared to the dansyl unit, making it a more privileged candidate as an LIP-sensor. Bidentate 3,4-HOPO was adopted for iron scavenging, which has an affinity to bind iron(III) as well as iron(II), leading to the partial Fe(II) chelation at a concentration <10 mM and, thus, capable of sensing Fe(II) levels. The design and preparation of compound 16 was done in isomerically pure form. A panel of probes was prepared where the Phe-residue in the tetrapeptide sequence central part (18, 21–22), at the nitrogen-terminus (19) or the carbon-terminal lysine (20) was replaced by the dual fluorescent probe. A structural analog of 20 was represented by compound 23, where a protected Fe-chelating moiety (17) was incorporated into the structure. Compounds 26 and 27 were designed by the activation of the glutamic acid residue, which was orthogonally protected on the solid support for the selective conjugation of either 1°- (24) or 2°-amine (25) having HOPO (Fig. 11). The sensitivity of mitochondria-targeted chelators towards iron(III) was determined by the incubation of buffered peptide solutions with the solution of Fe(NTA) complex with an increase in the concentration.
As the Fe(III) levels were increased, continuous quenching in fluorescence emission maxima was noted. Fe(III) chelation seems to play a role here, as the fluorescence intensity of peptide 23 was unaffected by the presence of Fe(III). A progressive decrease in fluorescence was induced upon increment of 1.5 mM Fe(HQ) to 18-treated cells as compared to untreated control cells. An initial addition of ten mM followed by a twenty mM increase of the deferiprone chelator imparts dequenching of the fluorescent signal, revealing that compound 18 is responsive to the addition of iron and removal from cells. The iron-binding peptides developed in this work impart a better design that can help in the selective delivery of iron probes to mitochondria and measure LIP levels under pathological and physiological conditions.
Four fluorescent probes, out of which three were coumarin-based and the remaining was fluorescein-based, have been reported.59 The chelating moieties are based either on 3-hydroxypyridin-4-one or 3-hydroxypyran-4-one. These probes (28–31, Fig. 12) imparted higher selectivity towards iron than other metals like Ni2+, Co2+, Mn2+, Zn2+ and Cu2+. Even though copper has a remarkable effect on quenching of fluorescence, its contribution to this study is likely negligible owing to its relatively low concentration in serum. Further, if the competition by Cu is a problem, the effect of copper on fluorescence quenching could be abolished by the inclusion of N,N,N′,N′-tetrakis(2-pyridylmethyl)-ethylenediamine in the assay. The addition of iron only leads to the quenching of fluorescence and does not result in a blue or red wavelength shift. Despite the selectivity of these probes for Fe(III), they were also highly responsive to Fe(II) as after combining with the probes, Fe(II) is immediately oxidized to form Fe(III) complexes. Apart from Fe(II) and Fe(III), quenching of fluorescence also occurred for other metals like Cu(II), Zn(II) and Co(II). No quenching was observed in the case of metal cations like Ca, Na, Mg, and K. Partial quenching of fluorescence occurred in the case of Zn(II), and it bound to the probe, diminishing quenching of fluorescence by Fe(III) as demonstrated by the slope of fluorescence quenching by Zn(II). As the iron affinity studies revealed, pyridone-based hexadentate ligands could compete with all iron from weak ligands like nitrilotriacetic acid and citrate. In fluorescein-based probes, the fluorescence intensity was quantitatively related to the concentration of iron, and the detection limit was 10−8 M.
Fakih et al. presented work on the development of Fe(III)-sensitive probe SF34 (32) having a 3,4-HOPO chelating moiety (Fig. 12) bearing selectivity and high affinity for Fe(III).60 The sensor in this work highlighted fluorescein as a fluorophore unit and possesses a maximum quenching of fluorescence up to 77.4% upon forming the neutral tris–Fe(III) complex at pH 7.4. The sensor exhibited the characteristic fluorescence properties of fluorescein in an iron(III)-free state. In a cell-free system at pH 7.4, the probe possesses an excitation maximum of 491 nm, and the emission maximum was at 514 nm. At the same time, an effective quenching of sensor fluorescence was noted upon iron(III) binding. The fluorescent probe's physicochemical and living cell properties, mainly accumulated in endosomal or lysosomal compartments, were evaluated. In vitro, responsiveness to changes in intracellular iron concentration and iron chelation power of clinically used Fe-chelators were examined using confocal microscopy and flow cytometry. In response to the non-fluorescent high-affinity Fe(III) chelators CP94 and salicylaldehyde isonicotinoyl hydrazone (SIH), dequenching kinetics of complex Fe(SF34)3 were monitored. CP94 showed a fluorescence recovery of 86.9%, while SIH, due to its higher Fe(III) affinity, could recover almost 92.3% of initial fluorescence intensity.
In another study, the iron chelators, which were fluorescent, were loaded into isolated rat hepatocytes, and the determination of the intracellular chelate iron pool of rat hepatocytes was based on the properties of HOPO fluorescent probes (Fig. 13). This iron pool quenched the fluorescence due to an excess in high-affinity iron chelator.61 This method can detect both the increase and decrease in the iron pool with high sensitivity at a single-cell level. Quenching of fluorescence occurred by adding the 8-HQ–Fe(III) complex but it was dequenched by adding an excess CP94. The intracellular probe concentration was found to be dependent on the partition coefficient in a way that the higher the hydrophobicity of the compound, the higher the intracellular concentration. The fluorescent chelator CP655 was found to be the most sensitive probe to monitor the chelated iron, as revealed by the increase in fluorescence by the addition of CP94.
A 3,4-HOPO-based tripodal ligand 33 (Fig. 14) was designed and developed by Chaves et al.62 The coordination behavior of ligand 33 was studied towards Gd3+ and Ga3+ through solution thermodynamics. Results from the potentiometric and spectrophotometric titrations suggested the formation of 1:1 metal–ligand complexes with the Ga3+ complex having higher stability than the Gd3+ complex (logβ = 14.3 for Gd3+ and logβ = 26.2 for Ga3+). From the DFT studies, the presence of two water molecules in the coordination sphere of the Gd3+–33 complex was confirmed, but the rate of water exchange in the Gd3+–33 complex is slightly lower than the Gd3+–34 complex which is possibly due to differing charges. However, the Gd3+–33 complex has high proton relaxivity. Further, the biodistribution studies indicate that the Ga3+–33 complex showed high in vivo stability and rapid renal elimination. Therefore, the Gd3+–33 and Ga3+–33 complexes can be potentially used for targeted labelled conjugates including MRI and 68Ga-PET diagnostic medicines respectively. Similarly, a new 3,4-HOPO-based ligand (34, Fig. 14) forms highly stable complexes with lanthanides (Ln3+ = La3+, Pr3+, Gd3+, Er3+, Lu3+) in aqueous solution.63 The potentiometric and spectrophotometric studies revealed the formation of 1:1 ligand–Ln3+ complexes with high stability. The logβ values increased from La3+ to Lu3+ and were found to be 22.86, 25.29, 26.35, 26.77, and 27.53 for the MLH species respectively. The DFT studies suggested the formation of Ln3+ complexes having 8 coordination numbers, six ‘O’ donors from ligand 34, and the presence of two water molecules in the coordination sphere. Also, 1H NMR and 17O NMR experiments confirmed the presence of two water molecules in the Gd3+–34 complex showing a high rate of water exchange. The affinity of ligand 34 was also checked towards zinc ions by solution thermodynamics and the results proved that the trans-metallation of the Gd3+–34 complex is not favored in the presence of Zn2+ ions. The Ln3+ complexes of ligand 34 can find their potential use as medical diagnostic probes, particularly the Gd3+ complex serving as an MRI contrast agent.
Two non-heme Fe(III) complexes, (35) and (36), were described for selective detection of inorganic phosphate employing 1,2-hydroxypyridinone ligands via the indicator displacement assay (IDA).64 Structures of probes 35 and 36 are shown in Fig. 15. The protection of open coordination sites to prevent μ-oxo dimer formation in aerated solutions was done by weakly coordinating fluorescein. A rapid and selective twenty-fold increase in emission intensity was noticed by the phosphate coordination accompanied by displacement of the fluorescein unit. Fig. 16(a) shows the fluorescence spectra of 35 toward phosphate and the increase in emission intensity. Both the receptors were exceedingly selective for phosphate over pyrophosphate and other competing anions, including halides, nitrate, arsenate, carbonate, and sulfate. The ligands allowed anion recognition by the Fe(III) center by its remaining open coordination sites. Probe 35 was found to be more selective over acetate and bicarbonate than 36. A higher selectivity for the targeted anion could not be achieved by a more sterically hindered recognition site.
Fig. 16 Fluorescence titration of (35) and (36) with phosphate (Pi): (a) fluorescence spectra of (35) with phosphate; (b) increase in the emission intensity.64 |
The detection limit of the Fe(III) receptors for 35 and 36 was reported to be 3.5 and 4.1 μM, respectively, enabling better phosphate detection of eutrophic water samples. Thus, these fluorescent Fe(III) probes offered an excellent ability to quickly monitor the phosphate level responsible for nutrient pollution in surface water.
Five new Eu3+ complexes EuIII-R-HOPO, where R is the amino acid lysine (37), glycine (38), serine (39), glutamic acid (40), and cystine (41), were designed by Huang et al. (Fig. 17), for sensing phosphate ions in water at pH 7.4.65 On adding ten equivalents of phosphate (H2PO4−/HPO42−) into each of the complexes, only three showed luminescence changes. The positively charged complex 37, neutral complex 38, and 39 enhanced luminescence by five-fold, twenty-fold, and thirty-six-fold, respectively [Fig. 19(a)]. This increase in the intensity is due to the replacement of water molecules, which are directly coordinated with metal center Eu3+ by the phosphate ions. The negatively charged complexes 40 and 41 showed no changes in the luminescence in the presence of phosphate ions. Thus, europium compounds 37, 38, and 39 are exciting candidates for detecting and remediating phosphate in complicated aqueous media due to their high selectivity, efficacy in water, and significant luminous response.
The increased level of phosphates in the human body leads to a biomedical illness known as hyperphosphatemia. The increased phosphate levels also pollute the environmental water, making it unfit for both aquatic and human life, emphasizing the necessity for developing robust receptors capable of effectively and selectively sequestering the anion from complicated aqueous systems. Four macrocyclic HOPO-based europium(III) probes containing either a cyclam (42), cyclen (43), TACD (44), or TACN (45) ligand cap were synthesized (Fig. 18) and reported for their sensing towards phosphate ions.66 Out of these four complexes, complex 44 has poor water solubility; hence, it could not be evaluated. The remaining three complexes were investigated initially using time-gated luminescence spectroscopy of the europium-centered emission. Complex 43 is an eight-coordination complex bearing two water molecules, while complexes 42 and 45 have nine-coordination around the Eu(III) metal having three inner sphere water molecules. Adding ten equivalents of the phosphate anions into the three complexes enhances the luminescence intensity to different extents at 615 nm (λex = 334–340 nm). Complex 43 loses one water molecule out of two, resulting in a 43–Pi ternary complex having a 1:1 stoichiometric ratio, whereas the other two complexes, 42 and 45, lose two water molecules and thus result in the formation of Eu(III)L–Pi2 complexes having 1:2 stoichiometric ratios. All three macrocyclics are selective against other competing anions, particularly bicarbonates and chlorides which are found in higher amounts in the blood; hence, these can be effectively used in treating hyperphosphatemia.
Huang and the group developed another group of 1,2-hydroxypyridonone-based europium(III) complexes (46–51) that were created (Fig. 20) to test their affinity towards various anions.67 Complexes 47 and 51 have broader and more flexible ligand caps, which favors the formation of nine-coordination with three water molecules in the inner sphere, whereas the stiffer caps of 46, 48, 49 and 50 favor eight-coordination around the Eu(III) center bearing two water molecules in the inner sphere. The luminescence behavior of all six complexes towards different anions was examined in the water medium, and studies revealed that probes 48 and 51 have a strong affinity solely for phosphate ions. In contrast, the other four complexes show no affinity for anions [Fig. 19(b)]. In probe 48 the two water molecules are replaced by two phosphate ions, resulting in a 10-fold increase in the luminescence intensity, whereas all three water molecules are replaced by the phosphate anion in probe 51, resulting in a twenty-fold rise in luminescence intensity. Thus, both the probes, 48 and 51, can be used for effectively sensing and sequestering the phosphate anions from the environment and biological systems.
Fig. 19 Changes in the integrated luminescence intensity of Eu-complexes (a) 37–41;65 (b) 46–51 (ref. 67) in the absence (white bars) and presence (grey bars) of ten equivalents of phosphate anion. |
The analysis of the Eu(III) centered complexes 48 and 51 has been further extended to understand the trend in phosphate affinity across the lanthanide series (La–Lu).68 The sensitization of Eu(III) complexes 48 and 51 through the hydroxypyridinone group formed brightly luminescent ternary complexes Eu(III)-2,2-Li-HOPO-Pi2 (52) and Eu(III)-3,3-Gly-HOPO-Pi3 (53) in the presence of two and three equivalents of phosphate anions respectively. Herein, the authors have developed a series of lanthanide complexes composed of the same ligand and different lanthanides viz., Ln(III)-2,2-Li-HOPO (Ln-1) and L(III)-3,3-Gly-HOPO (Ln-2) where Ln(III) is La3+ to Lu3+. To establish the trend towards phosphate, the receptors Ln-1 and Ln-2 are added one after another from La–Lu to probes 52 and 53, respectively. From the data, it is revealed that in both cases, the affinity of the receptors increases towards the phosphate anion as we go down the series. Hence, the lanthanides coming after Eu3+, having a smaller size than Eu3+, form a more stable complex with the HOPO-based ligands and are capable of sequestering the phosphate anion from both the Eu3+-centered complexes 52 and 53 and water molecules again take the position of phosphate in 52 and 53 resulting in an overall decrease in the time-gated luminescence intensity due to the ternary complexes 52 and 53. Overall, the authors conclude that the late rare earth lanthanides have higher potential for anion sequestration, particularly lutetium(III), seven orders of magnitude greater than lanthanum(III).
Considering the biological compatibility along with low molecular weight, a mono-substituted naphthalene fluorescent platform was used to covalently bind a chelator moiety, resulting in the development of two new 3,4-HOPO/naphthalene conjugates 54 and 55 (Fig. 21).69 The naphthalene skeleton is marked in many clinical drugs, as is using naphthalene-based fluorophores to prepare fluorescent sensors. The X-ray analysis confirmed the structures of both compounds as synthesized using traditional heating and microwave irradiation methods. Also, the tautomeric keto forms can be obtained in solution and when the base is present. The tautomeric form and the distance between the fluorescent and chelating functions significantly impact the fluorescence properties of the ligands. Further, 54 was designed such that the fluorophore and the chelating moiety were directly attached through the N atom of the pyridinone ring, while in 55, the same functions were separated by the –CH2CH2CONH– bridge, thereby generating a more flexible structure. Fluorescence was observed at 450 nm in 54 dihydroxypyridinium in DMSO and ACN, while the keto form showed fluorescence at 365 nm. 54 showed non-fluorescent properties in ethanol, methanol, and water, revealing the fluorescence of 54 in aprotic solvents only. In the case of 55, the keto form did not exhibit fluorescence, while its dihydroxypyridinium was fluorescent at 340 nm only in DMSO. Therefore, 54 displayed good fluorescence properties and the quenching in fluorescence was observed with variable concentrations of Zn2+, Cu2+, and Fe3+, marking its applicability as an ion sensor. The dependency of fluorescence properties on the naphthalene and the pyridinone distance was thus shown by this distinct fluorescence behavior.
The development of probes is significant for detecting and quantifying cyanide ions due to their high toxicity to the environment and humans. Huang's group presented the development of a luminescent europium probe (EuIII-Lys-HOPO, 56) that responds to CN− directly in water (Fig. 21).7056 incorporated a 1,2-HOPO-based sensitizer and a wider ligand cap to favor anion coordination by increasing the number of inner-sphere water molecules. The lysine side arm was used to improve the hydrophilicity of 56. Characteristic emission spectra with a primary peak at 615 nm were observed when 56 was dissolved in pure water. A nine-fold increase in luminescence intensity in the presence of CN− is observed in the case of 56 and is found to be hugely selective over other competing anions of environmental relevancy in the presence of Ca2+. This turn-on response was never observed earlier in the case of CN− probes that function in water. The probe also showed a twelve-, seven-, and two-fold increase for phosphate, carbonate, and fluoride at pH 10.
Nevertheless, it does not bind Cl−, Br−, I−, CH3CO2−, SO42−, and NO3−. Adding a soluble Ca2+ salt such as CaCl2 can eliminate the interference from the competing ions. The competing anions did not affect the CN− concentration determination in the presence of Ca2+, as demonstrated by the nine-fold increase in metal-centered luminescence upon subsequent addition of a hundred equivalent of CN−. Fig. 22 depicts the selectivity of 56 towards various environmentally relevant anions and the illumination of 56 solutions in water.
Fig. 22 a) Selectivity of 56 to various environmentally relevant anions. b) Picture showing solutions of 56 in water with corresponding anions and CaCl2 (100 equiv. each) under UV illumination.70 |
In another work, the interaction between the 1,2-HOPO-based hydroxypyridinonate chelator (58) and Au3+ in solution was characterized, where complexation of the cation with the ligand was observed even at pH 2.2 (acidic) when mostly HOPO binding moieties were protonated.73 The reported octadentate hydroxypyridinone chelator acts as a therapeutic agent due to non-cytotoxicity at desired dosages and high affinity for f-block elements. The thermodynamic properties of the ligand and Au3+ complexes were explored, and the growth of gold nanoparticles by the chelator under specific pH conditions was demonstrated. The complex formation was followed by the reduction of Au3+ to Au0 with increased pH. At pH 4.9, monodisperse AuNPs were obtained with the 1,2-HOPO-based ligand, which acts as a stabilizing and reducing agent. The sensitization properties of the chelator were exploited for confirmation of the presence of chelator on the metal surface. An emission band at 618 nm was observed for the complex formation between Eu3+ and the chelator. The UV-visible spectrum confirmed the aggregation of the particles upon the addition of Eu3+. The chelator can act as both a stabilizing and reducing agent in the growth of particles, as enhancement in fluorescence occurs upon aggregation of particles, and quenching of the emission of fluorophores occurs due to the AuNPs located on their surface. Gold nanoparticle selective aggregation confirms the selectivity towards f-block elements over the lighter elements as the chelator on the Au surface preserved only lanthanides. This new colorimetric assay was reported to be able to reach the detection levels necessary for quantifying lanthanides in industrial waste using the inhibition of the growth of particles by lanthanide elements. This synthesis of gold nanoparticles with a chelator having a good affinity for f-block elements on the surface can pave the way for prospects in therapeutics and biosensing.
Gadolinium ions chelated with linear or macrocyclic ligands are the most common contrast agents employed in about 25% of all MRI processes to enhance image contrast. Several disorders, like consequential nephrogenic systemic fibrosis in patients with renal dysfunction and gadolinium accumulation, have been observed in the healthy person's brain and are related to gadolinium-based contrast agent (GBCA) administration.74,75 Hence, the tracking of contrast agent excretion through urine after imaging procedures by analytical techniques is necessary for patient safety. Pallares et al. reported a luminescence assay to detect GBCAs in urine based on the quenching of probe, Eu3+–58, which needs only ten min incubation before measurement.76 The octadentate chelator, comprised four 1,2-HOPO subunits linked to a central spermine scaffold, was explored for f-block element decorporation. It was investigated for bioassay development emission of several lanthanides, including Eu(III), which can be sensitized by the 1,2-HOPO group. The formation of the Eu3+–58 complex was prevented by gadolinium-based contrast agents, thereby quenching its emission.
Meanwhile, the chelator Eu(III) complexes sensitized the emission without a contrast agent. The performance of the assay was successfully tested for urine samples of mice. Fig. 24 depicts the optical response of the Eu3+–58 complex.
Fig. 24 (a) Absorbance and emission intensity (in a.u) of Eu(III)–58 (20 μM). (b) Optical response noted for 20 μM complex to 50 μM Gd3+ and other cations and subsequent (c) quenching of emission intensity at 620 nm.76 |
A new library of tetrameric peptoid ligands (60–63, Fig. 23) containing HOPO or catecholamide chelating moieties linked via an ethylenediamine bridge for chelation of f-block metals was synthesized by Ricano and the group using incorporation of high-affinity metal binding moieties to peptoid scaffolds.77 The mixed ligands sourced from the spermine scaffold were also synthesized for comparison. They proposed to lift the ligand synthesis process by incorporating custom-made sub-monomers with better binding properties of f-elements. Coordination studies based on luminescence for all the ligands were carried out using Tb3+ and Eu3+, along with determining stability constants with Eu3+ for selected chelators. Considerable differences in photophysical properties and stability of the complex may result from slight variations in ligand structure, as revealed by the studies. Both ligands had more affinity for Ln3+ metals with stability constants greater than 1029 for Eu(III). 1,2-HOPOs have a higher attraction towards lanthanides and were found to sensitize Eu3+ luminescence. In this work, 1,2-HOPO ligands substitute a nucleo-base pair in a ten-base-pair peptide nucleic acid (PNA) duplex, contributing a solid binding site for Eu3+ (Fig. 25).78 1,2-HOPO was incorporated into a PNA monomer, which was used to create the binding site for Eu3+ in a duplex of peptide nucleic acid. The versatile catalytic and luminescence properties of lanthanides and PNA's affinity to bind towards DNA and RNA made them more fascinating to design optical and MRI sensors. One or three lysine residues were contained at the carbon end of each oligomer (Lys PNA-HOPO or Lys3 PNA-HOPO, respectively) to advance the solubility of PNA. The binding of Eu3+ to ligands containing the 1,2-HOPO moieties of the Lys PNA-HOPO duplex was supported by luminescence studies. When the HOPO unit of Lys PNA-HOPO in solutions was excited at λ = 318 nm, the Eu(III) emission spectrum with a noticeable 5D0 → 7F2 emission peak at 614 nm was observed. The spectral data concluded the sensitization of Eu3+ luminescence by 1,2-HOPO.
Fig. 25 Structures of bidentate ligands 1-hydroxy-2-(1H)-pyridinone-2-carboxylic acid propyl amide (Pr-1,2-HOPO) and PNA-HOPO. |
Moore and co-workers reported the unique luminescence properties of 1,2-HOPO-based Eu3+ sensitizers.79 Two octadentate derivatives bearing a pendant amine or a carboxylate group were introduced to allow the conjugation of the complexes with biomolecules of relevance. They found that the photophysical properties of the complexes are greatly affected by the protonation of apical amines. Hugely effective emission with good stability (pEu ∼ 20.7–21.8) at pH 7.4 in these complexes was observed in an aqueous solution. Also, at pH ∼ 6, the observed intensity is quite decreased due to quenching, which involves the amine backbone protonation, making these complexes bright candidates for applications in homogeneous time-resolved fluorescence assays. The practicability of their utility as long-lived luminescent tags in a bioassay was also demonstrated.
In another work, a series of tetradentate 1,2-HOPO derivatives (64), (65), and (66), as Eu(III) sensitization geometric components have been examined (Fig. 26).80 Notable differences were observed in the luminescence quantum yield upon alteration of the bridging unit. At pH 7.4 in aqueous solutions, a considerable effect on quantum yield from 21% (aliphatic bridged derivatives) to 6% for [Eu(64)2]−, apart from affecting the significant triplet excited state, was observed upon the use of the aryl moiety as a bridge. A considerable quantum yield quenching was observed for [Eu(66)2]− as compared to [Eu(65)2]− which may be attributed to one water molecule in the inner sphere bound to Eu3+ while for [Eu(64)2]−, the low quantum yield may be due to ineffective sensitization of Eu3+. Also, adequate sensitization along with valuable metal-centered emission was shown by the [Eu(65)2]− derivative.
1,2-HOPO-based ligands form relatively stable complexes with Eu(III) in an aqueous solution and have proven to be effective sensitizers towards Eu(III). Moore and his group studied the synthesis, structural, and photophysical properties of various Ln(III) complexes formed by using two different bis-bidentate ligands (67) and (68) with 1,2-HOPO-based chelating moieties (Fig. 27).81 Fine transitions from the 5D0 excited-state (metal-centered) to 7FJ ground state multiplet reside in the characteristic emission spectrum of the Eu3+ complex.
For transitions J = 1, 2, 3, and 4, maximum intensities were observed at 593, 612, 652, and 702 nm, respectively. The quantum yield for the [Eu(68)2]− complex was comparable to [Eu(67)2]− at 7.4 pH. Luminescence lifetime was acquired for both complexes viz., [Eu(67)2]− and [Eu(68)2]− which were shorter than the measurement of the corresponding solution, which can be ascribed to the self-quenching mechanism in the close-packed solid system. On UV irradiation, a light pink emission was observed for Sm3+ complexes of 67 and 68, showing metal-centered luminescence. The most intense transition at 646 nm corresponds to that from 4F9/2 to 6H9/2. The sensitization towards other Ln cations, like Tb3+ and Dy3+ for ML2 complexes of 67 and 68, was also measured, but metal-centered luminescence was observed only in the case of Eu(III) and Sm3+ complexes. Moore et al. have reported the synthesis and photophysical properties of 69 and 70, as shown in Fig. 27, which employed the 1,2-HOPO chelator as a Eu3+ sensitizer.82 These ligands were found to be thermodynamically stable and exhibit remarkable photophysical properties. 69 was developed as a model for 70, which, upon dilution in aqueous solutions, forms a complex (ML) with better stability, imparting the vital feature required for the applicability of these complexes towards biological assays. The [Eu(69)2]− complex formed by tetradentate (69) is stable up to 7 × 10−9 M concentration in an aqueous solution. The octadentate ligand (70) imparts improved stability as the [Eu(70)]− complex upon additional dilution to a limiting concentration of 5 × 10−17 M, the limit of which is outside the minimum detectable concentration of major fluorimeters. Overall, metal-centered luminescence was reduced due to the presence of single coordinated water for [Eu(70)]−. The electronic absorption spectrum of [Eu(70)]− has a slight red-shift in λmax at 341 nm, which was lower than the [Eu(69)2]− complex. The luminescence spectrum of [Eu(70)]− is representative of that of the Eu3+ cation in a low-symmetry environment. Fig. 28 represents the electronic spectra of the complex.
Fig. 28 Figure showing the absorption spectrum in the left and the steady-state emission spectrum in the right.82 |
Daumann and co-workers presented the ligand geometry effect on the photophysical properties of Eu3+ and Sm3+ 1,2-HOPO complexes in an aqueous solution.83 They prepared a series of ten tetradentate 1,2-HOPO ligands (71–80, Fig. 29) along with analogous Eu3+ and Sm3+ complexes with eight coordination. The authors reported the effect of the change of linker backbone length between two bidentate 1,2-HOPO chelates and the resulting photophysical properties of the complexes. A [LnL2]− geometry resulted in the binding of 1,2-HOPO tetradentate ligands to Ln3+ (2:1). The results interpreted the significance of geometry optimization of the ligand around the lanthanide center for maximizing the transfer efficiency of energy to the Ln center from the organic chromophore for enhancing Φtot. The fair change in brightness of these mentioned species could be attributed to the effect of the geometry of the chromophore, changed by the aliphatic linker length around the Ln(II) center.
Fig. 29 Structures of tetradentate 1,2-HOPO ligands reported by Daumann and co-workers.83 |
Another group reported a 3,4-HOPO functionalized carbon quantum dots-based probe (HOPO-CQD) for quick detection and monitoring of uranyl ions.84 The excellent detection performed by this fluorescent probe can be demonstrated by a limit of detection (6.53 ppb) and a shorter response time (30 s). The photoluminescent property of this chemosensor was investigated by fluorescence spectroscopy measurements, which revealed the highest fluorescence intensity at pH 4. The fluorescence intensity of HOPO-CQD was remarkably quenched in the presence of UO22+ as compared to other ions present. This noteworthy quenching was attributed to the uranyl-specific aggregation of HOPO-CQD resulting in the phenomenon known as aggregation-caused quenching. Also, the inner filter effect was discovered due to the partial overlapping of the fluorescence spectrum with the absorption spectrum of uranyl ions, thereby contributing to the fluorescence quenching.
Other than the HOPO-based chelators, there are some environment-friendly hybrid materials reported for the fluorescence sensing of metal ions. A fluorescent microcapsule probe 81 developed using sporopollenin, ethylenediamine, and BODIPY was reported for selectively sensing Cu2+ ions in water:ethanol medium (70:30 v/v).85 Among various metal ions, the fluorescence of probe 81 was quenched in the presence of Cu2+ ions only, with a detection limit of 0.773 μM. The micro capsule 81 is also able to absorb the Cu2+ ions and hence can effectively remove them which makes the probe exceptional. Similarly, another hybrid material 82 (Fig. 30) was developed based on BODIPY and f-silica gel for selective recognition of Cu2+ ions in an aqueous medium.86 In the presence of Cu2+ ions, a 9-fold quenching in the fluorescence of probe 82 was observed. The detection limit was found to be 4.63 μM. Also, the Langmuir adsorption isotherm was used to study the adsorption behavior of Cu2+ ions, resulting in a maximum capacity of 19.920 mg g−1. These results suggest that the hybrid material 82 can be used both as a sensor and an adsorbent for the detection and removal of Cu2+ ions from environmental wastes.
HOPO | Hydroxypyridinone |
NTBI | Non-transferrin bound iron |
GBCA | Gadolinium-based contrast agents |
LIP | Labile iron pool |
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