Interactions of non-polar and “Click-able” nucleotides in the confines of a DNA polymerase active site

Abstract

Modified nucleotides play a paramount role in many cutting-edge biomolecular techniques. The present structural study highlights the plasticity and flexibility of the active site of a DNA polymerase while incorporating non-polar “Click-able” nucleotide analogs and emphasizes new insights into rational design guidelines for modified nucleotides.