Perspective
Finding a vocation for validation: taking proteomics beyond association and location
We discuss modern quantitative proteomics tools and underlying experimental design principles, to help readers chose suitable methods and workflows, as well as analyses and functional validation of the resulting data and targets.
Communication
Chemoproteomic mapping of human milk oligosaccharide (HMO) interactions in cells
Human milk oligosaccharides (HMOs) are a family of unconjugated soluble glycans found in human breast milk that exhibit a myriad of biological activity.
Communication
Quantitative profiling of PTM stoichiometry by resolvable mass tags
Post-translational modifications (PTMs) play important roles in modulating the biological functions of proteins.
Paper
Predicting small molecule binding pockets on diacylglycerol kinases using chemoproteomics and AlphaFold
We provide a family-wide assessment of accessible sites for covalent targeting that combined with AlphaFold revealed predicted small molecule binding pockets for guiding future inhibitor development of the DGK superfamily.
Paper
Photoreactive bioorthogonal lipid probes and their applications in mammalian biology
This review summarizes the recent advances in the development of photoreactive bioorthogonal lipid probes, and the use of these lipid probes in mapping diverse biological pathways in mammalian cells using emerging chemoproteomic approaches.
Paper
Chemical proteomic analysis of bile acid-protein targets in Enterococcus faecium
Chemoproteomics of bile acid-protein targets reveals a bile salt hydrolase in Enterococcus faecium.
Paper
A peptide-crosslinking approach identifies HSPA8 and PFKL as selective interactors of an actin-derived peptide containing reduced and oxidized methionine
A peptide crosslinking approach facilitates the identification of proteins that selectively interact with actin-derived peptides containing oxidized and reduced methionine residues.
Paper
The covalent reactivity of functionalized 5-hydroxy-butyrolactams is the basis for targeting of fatty acid binding protein 5 (FABP5) by the neurotrophic agent MT-21
In this work, it is shown that an N-acyl hemiaminal motif present in many natural products can function as an electrophilic centre, mediating covalent reactivity in biological systems, reacting with both thiols and amines.
About this collection
This collection, Guest Edited by Dr Keriann Backus (UCLA, USA) and Dr Stephan Hacker (Leiden University, Netherlands), highlights work on applications of chemoproteomics to study the targets and off-targets of covalent and non-covalent inhibitors, to study the reactivity of amino acids in the proteome, to develop new reactive groups for photocrosslinkers, covalent inhibitors and protein labeling as well as to study post-translational modifications and cofactor binding proteome-wide.