Themed collection Analytical Methods HOT Articles 2024

Nonenzymatic biosensors for sports wearables face challenges in sensitivity & specificity. Antigen–antibody, aptamer, & molecular imprinting strategies are explored, enhancing performance via probes, nanostructures, multi-targets, & microfluidics.

Typical polyoxometalate-based electrochemical sensors and their applications in environmental monitoring, food safety and biomedicine in the last three years are summarized in this review.

This short review presents the latest advances in the field of electrochemical biosensors, focusing particularly on impedimetric biosensors for the direct measurement of analytes.

In this review, we primarily summarized four temperature-response strategies. Then, we further analyzed the chemical modifications and biological applications of the probes. Finally, we have provided a prospective on the future development of probes.

This review focuses on electrochemical sensors for the determination of AP in the past ten years. In particular, the design and construction of AP sensors are discussed from the perspective of non-enzyme materials and enzyme substances.

A review with 93 references describing various 3D printing approaches that have been used to create microfluidic devices containing electrodes for electrochemical detection.

Recent advancements in analytical methods for vitamin D and its metabolites have greatly improved to accurately determine and quantify these substances in various sources, including biological fluids, fungi, and both natural and fortified foods.

Detection strategies of cancer biomarkers with various techniques based on the CRISPR-Cas system.

Cardiac biomarkers and their connection to cardiovascular diseases.

The paper gives overall perspective of the promising field by reviewing diversity of functional sensing fibers/textiles and hopes researchers track the progress of flexible sensing fibers/textiles for applications in personal healthcare system.

Substantial progress in the technological development of mass spectrometry-based top-down proteomics enabled broad biomedical applications for bettering our understanding of proteoform function in modulating diseases and development.

Aptasensors for thrombin, which feature a sandwich-type configuration, hold great promise in terms of their potential applications in diagnostics and therapeutics.

MXene-based luminescence sensing strategies include fluorescence, electrochemiluminescence and chemiluminescence sensors. Their performance are summarized, and their challenges and application perspectives are discussed.

Xylazine represents an increased threat to the recreational drug market.

A novel surface charge induced electrospray ionization is proposed due to its features of no contact between sample solution and metal electrode, fast analytical process (less than 40 s), and high analytical sensitivity.

A diffusion cell setup within an NMR tube enables automated drug permeability measurements. Challenges, modifications, and additional applications are discussed.

A novel dicyanoisophorone-based colorimetric fluorescent probe 3 has been prepared for recognizing peroxynitrite in aqueous conditions.

A multifunctional rhodamine derivative (BBS) was designed and synthesized as a colorimetric and fluorescence turn-on probe for the selective detection of copper (Cu²⁺) and hypochlorite (OCl⁻) in aqueous media.

A multi-detection strategy based on branched DNA nanostructures was developed to achieve the simultaneous high specificity identification and highly sensitive fluorescence responses to HBV antigen and HBV DNA.

A fluorescent aptasensor coupled with target-triggered aptamer hairpin switch and nicking enzyme-assisted signal amplification for digoxin detection in plasma for therapeutic drug monitoring.

We developed a rapid colorimetric method for ENR detection with a 1.72 nM LOD that is applicable to various samples, including water, milk, serum and urine, and integrated it with a smartphone platform for convenient on-site detection.

A glassy carbon electrode modified with porous boron-doped carbon nitride and gold nanoparticles was prepared. A label-free electrochemical method for the immunoassay of vascular endothelial cadherin was developed.

K. pneumoniae strains from clinical samples were cultured and string-tested, among which ten hypermucoviscous strains and ten regular strains were randomly collected for SERS spectral analysis.

Glycosphingolipids are glycolipid complexes formed by an oligosaccharide chain covalently linked to a ceramide backbone and play important roles in the occurrence and metastasis of lung cancer.

Schematic of the process for the fabrication of the Fe/PDA-C/CC electrode.

A simple turn-off fluorescent probe DHP is designed and synthesized for the sensitive and selective detection of Cu²⁺. The probe DHP exhibits good anti-interference performance against Cu²⁺ in the presence of multiple metal ions.

Bimetallic gold/silver functionalized carbon sphere-based electrochemical immunosensor demonstrates significant integrated performance in the detection of CYFRA 21-1 and the protection of information.

ATP can be quantified based on the variation of the fluorescence intensity of DNA-AgNCs, caused by the structural change upon cleavage by DNAzyme.

An ultra-sensitive photoelectrochemical sensing platform for profenofos detection based on the inhibition of catalase activity was prepared in this work.

In this work, we proposed a simple way to enhance underwater LIBS signals with a solid substrate-assisted method, promoting the development of compact marine in situ LIBS sensors with low power consumption, while ensuring high detection sensitivity.

Microregion pH of semi-solid preparations such as ointments, creams, gels can be revealed by pH-sensitive surface enhanced Raman scattering microtips.

The nano-Schottky-junction-designed Pd/SnO₂ sensor enables ultrasensitive room-temperature detection of H₂, with a low limit of detection of 1.6 ppb.

An Fe₃O₄-Au nanoparticle-based LFA was developed for simultaneous detection of multiple influenza virus nucleic acids. This LFA showed good accuracy and satisfactory sensitivity, and could serve as a portable method for early influenza detection.

A bisphenol A based chemosensor (Me-H₄L) is developed for the detection of Al³⁺ in animal cells (HepG 2) and plant roots.

Canine parvovirus (CPV) is one of the main pathogens causing toxic diarrhea in Chinese dogs, is the cause of a large-scale epidemic of dogs, and poses a great threat to the dog industry in China.

A method for measuring the action potential of cardiomyocytes using an improved AFM conductive probe as a nanoelectrode. The beating force and action potential of cardiomyocyte were recorded simultaneously using two different methods.

This study investigates the potential and complementarity of high-throughput multipulse and multidimensional NMR methods for metabolomics.

A novel dual-model photoelectrochemical/electrochemical sensor based on Z-scheme TiO₂ disks/methylene blue for the detection of Kana was developed.

This work reports a DNA aptamer selected with the cell-SELEX method, which specifically binds to esophageal cancer cell line TE-1 with great affinity and specificity both in vitro and in vivo.

Taking into account the drug resistance of antibiotics, teicoplanin has been banned in the veterinary field.

Fumonisin B1 (FB1) is a mycotoxin, a water-soluble metabolite produced by Fusarium cepacia, which mainly contaminates grain and its products and is acutely toxic and potentially carcinogenic to certain domestic animals.

A photoelectrochemical immunoassay was devised to detect CA-125 employing enzyme-catalyzed precipitation on hollow porous In₂O₃ nanotubes with CdS nanoparticles.

There is an increasing demand for an inexpensive, quick, accessible, and simple method for the detection of urinary tract infection (UTI) together with the antibiotic-resistance profile of the infection-causing bacteria.

An efficient and practicable biosensor to detect morphine and codeine was fabricated by means of 2-amino-5-mercapto-1,3,4-thiadiazole functionalized gold nanorods (AMTD-AuNRs) in aqueous solution.

A polymer nanogel with oxidase-like activity was firstly synthesized. Furthermore, a colorimetric-fluorescence dual-mode detection platform was constructed to detect ascorbic acid. The platform can be used in real samples with satisfactory results.

oxTMB and Ce³⁺-GSH-Au NCs, prepared by a cascade reaction, were used to achieve the dual-signal readout of the nerve agent sarin.

Methyl parathion, a highly toxic, efficient, and persistent organophosphorus pesticide, is widely used in China.

A FRBN-B probe was used to detect BChE activity in both normal and NAFLD zebrafish liver tissues by cleaving the cyclopropanecarbonyl group, resulting in an increased fluorescence signal.

Effective quantification of 3-acetyl-11-keto-β-boswellic acid in nanocarrier systems, in vitro drug release samples, and skin matrices using the RP-HPLC method.

Easy-to-use methanol detector was tested in an ISO-standardized trial with 17 independent participants, showing comparable accuracy to gold-standard gas chromatography.

We designed and prepared probe W-1 for the detection of H₂O₂.

A mitochondria-specific NIR fluorescence probe (CMBT) has been constructed for dual-detection of sulfur dioxide (SO₂) and viscosity with high selectivity and sensitivity in living cells and mice.

The mechanism of the effect of sample preparation methods on the signal was explored through an in-depth study of the plasma density and temperature.

To thoroughly understand ferroptosis's biological functions in living cells, it is crucial to investigate the polarity variations that occur during this unique Fe(II)-facilitated oxidative type of cell death.

Strong cation exchange – mass spectrometry method was optimized for intact analysis of sialylation glycoproteins.

Experimental decoupling of the effects of plasmon resonance energy transfer (PRET) and metal-enhanced fluorescence (MEF) within the same nanometal–fluorophore pair is fascinating but challenging.

A photoelectrochemical (PEC) biosensor for silver ion (Ag⁺) detection was constructed based on Zn–Co doped C and CdS quantum dot (CdS QD) nanomaterials.

The colorimetric detection of cysteine (Cys) from whole blood using a dried PVP–AuNP paper surface as a colorimetric probe.

The potential of HPTLC was studied to analyse the inhibitory properties of ATIs from wheat, spelt, and einkorn flours. It provided information on changes in individual saccharides or peptides and was more reliable than conventional in-vial assays.

We have designed a polarity fluorescent probe (DCI-Cou-polar) with high selectivity for diagnosing cancer.

We report PA-γ-PGA-modified asymmetric nanochannels that enable ultra-sensitive detection of Ni²⁺ (limit of detection is 1 × 10⁻⁸ M).

Amidoxime adsorbent was synthesised by graft polymerisation. The adsorbent was efficient for collection of U in seawater for both the adsorption and desorption steps, thus affording a new strategy for measurement of U isotopes.

The 3-NPH-labeled sialic acids produce a high abundance of reporter ions, which can improve the accuracy of quantitative analysis of O-acetylated species.

A lateral flow strip based on a truncated aptamer for on-site detection of homocysteine in human serum.

Herein, a magnetic bead-based chemiluminescence assay is reported to detect type IV collagen (col-IV) in serum samples.

A novel pH-response fluorescent probe MP for the detection of mitophagy process via tracing colocalization coeffcients was developed.

An ECL microsensor integrating RCA magnification and in situ DNA-templated AgNC generation for sensitive detection of cellular ATP.

A low-conductivity sample buffer plug was electroosmotically injected, and then the FASI-EKS was conducted at a reversed voltage polarity. When the conentrated analyte region arrived at the capillary inlet edge, a normal voltage was applied for separations.