Themed collection 150th Anniversary Collection: Mass Spectrometry

We outline the achievements and remaining challenges of mass spectrometry-based tissue spatial proteomics. Exciting technology developments along with important biomedical applications of spatial proteomics are highlighted.

NP1 digestion coupled with UHPLC-HRMS can provide valuable information on stereochemical comparability between phosphorothioate oligonucleotides by examining digestion fragments.

The first calculations of the similarity and correlation of lipid images between MALDI and WALDI for evaluating a common ground truth in MS imaging.

In this study, we propose a novel ion formation simulation method for electrospray ionization (ESI) and atmosphere pressure interface (API).

A submicron emitter tip and relay ESI configuration enable the measurement of the ESI flow rate in the femto liter per minute (fL min⁻¹) range.

A new method for determining the concentration of elements in single cells by the SC-ICP-TOF-MS method based on a metal-containing stain as a cell volume proxy has been developed and validated.

The effective electric field radius is a fundamental parameter of ion traps, and it has a significant influence on ion-trapping capability, signal intensity, mass range and some other properties of the ion trap.

A combination instrument would be a significant cost saving in an analytical laboratory, and the construction of such a device appears to be possible.

Instead of possessing a fixed conformation, it is found that a calmodulin molecule actually jumps between two very distinct conformations. The binding of Ca²⁺ metal ions did not affect the structural dynamics of calmodulin.

Optimization and evaluation of thermal evaporation as a technique for silver deposition in MSI of cholesterol.

It is possible to inhibit the rates of ion-ion reactions involving high mass ions via the selective separation of reactant ion clouds. A comprehensive model for predicting conditions for successful ion parking with illustrative data are provided.

Multiple ion isolation and accumulation events reduce abundant chemical noise in MALDI imaging mass spectrometry experiments leading to improvement in dynamic range and limit of detection.

Samples collected from brain of living subjects are segmented to nanoliter droplets and infused to the mass spectrometer. Temporal resolution for in vivo concentration dynamics of key neurotransmitters of 10 s is achieved. Created with Biorender.com.

A recently developed method enables the loss of individual charges from 1 to 10 MDa salt clusters to be resolved using charge detection mass spectrometry. This technique is well suited for investigating the mechanics of late stage ion formation.

A selected ion monitoring (SIM) approach combined with high resolution mass spectrometry is employed to identify and distinguish SARS-CoV2 recombinant virus variants by means of mutated peptide segments in the surface spike protein.

Robust EV isolation from CSF by EVTRAP and downstream in-depth phosphoproteome analysis in PCNSL were performed, demonstrating its potential applications.

MobCal-MPI-2.0, the first major update from its predecessor, calculates an ion's CCS and high-field mobility with optimized accuracy and efficiency.

Early and accurate diagnosis of gastric cancer is vital for effective and targeted treatment.

The mechanism of in-source nucleoside fragmentation in ESI-MS was revealed, leading to nucleoside analysis with an enhanced response and less ambiguity.

This work describes the development of a new approach to measure drug levels and lipid fingerprints in single living cells.

The mechanism of enhanced photocatalytic indole cation-radical cyclization in confined space is examined by coupling a nanopipette reactor with in situ nano-electrospray mass spectrometry (nanoESI-MS).

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with a high mortality rate.

Internal fragments generated by top-down mass spectrometry can increase sequence coverage, localize disulfide bonds, and determine disulfide connectivity of disulfide-containing proteins.

The present work describes the application of an in-house developed 3D-printed open port probe (3DP-OPP) with differential ion mobility spectrometry (DMS) mass spectrometry.

Insoluble carbonaceous deposits were grown in internal combustion engine components and interrogated by OrbiSIMS depth profiling, and we uncovered the composition and proposed time resolved growth mechanisms of these materials.

LC-MS/MS enables the separation and relative quantitation of cyclic and tadpole architectures of poly(glycidyl phenyl ether)s synthesized via ring-opening zwitterionic polymerization (ZREP).

Charge transfer dissociation-mass spectrometry (CTD-MS) enables the differentiation of L- and D-epimers of Asp and isoASP amino acids in peptides.

Natural lignin was firstly applied as a dual-ion-mode MALDI matrix for small molecules, which successfully identified over 30 different small molecules and six kinds of representative molecules were quantified with a good linear response.

Complete inline reduction of NISTmAb disulfide bonds using an electrochemical cell coupled to LC-MS analysis, reduction is carried out without the use of traditional reducing agents or enzymes.

Ion mobility mass spectrometry (IM-MS) supported by theoretical calculations allows describing the conformational changes of macrocycle receptors upon anion binding.

The charge state dependence of 213 nm ultraviolet photodissociation mass spectrometry in top-down proteomics is investigated revealing the effect of side chain and amide backbone protonation on cleavage sites.

TiN nanopillar was applied as a robust analytical substrate for the laser desorption/ionization mass spectrometry of various types of analytes with a wide mass range. Numerical simulation validated the heat confinement effect of the pillar shape.

We present a one-step fabrication method for a new multiplexed electrospray emitter with nine parallel micronozzles.

Native mass spectrometry allows the screening of possible protein bioreceptors for perfluorinated alkyl substances.

Raman and infrared are used to measure dynamics in bacterial metabolism by employing stable isotope probing with temporal acquisition. The work is complemented by SIMS for confirmation of ¹³C incorporation into bacterial biomass.

We developed a tandem enrichment strategy combining metabolic labeling-based RNA tagging for selective RBPs purification and HILIC-based enrichment for simultaneous O-GlcNAcylation and phosphorylation profiling.

Microfluidic chip and electrospray ionization mass spectrometry based bacterial antimicrobial resistance analysis has been implemented rapidly and accurately.

Mobilograms of in situ ESI 3-pyridinylboronic acid tagging of isomaltose in the positive or negative mode.

Next-generation derivatives for photodissociation-mass spectrometry for fatty acids generating photoproduct yields of up to 97% at 266 nm.